CAJ | 학술논문

背景：微弧氧化技术可改善钛或钛合金的表面特征。 　　目的：研究纯钛表面微弧氧化涂层的表面性能及其对MC3T3-E1细胞早期黏附、增殖及成骨能力的影响。 　　方法：将46个直径10 mm、厚度2 mm 圆盘状纯钛试件分为实验组和对照组。实验组置于含0.02 mol/Lβ-甘油磷酸二钠盐及0.2 mol/L乙酸钙的电解液中进行微弧氧化处理，对照组对试件进行机械抛光。扫描电子显微镜观察试件表面形貌，X 射线能谱分析检测涂层表面钙磷比，X 射线衍射分析检测涂层晶相构成。将MC3T3-E1细胞接种在两组试件表面，1，2，4 h电镜下观察细胞形态，在2，4，7 d通过CCK-8方法检测细胞增殖，并于7，14 d检测碱性磷酸酶活性。 　　结果与结论：经微弧氧化处理后，钛表面形成粗糙多孔的钙磷涂层，微弧氧化涂层主要元素为Ca、P、O及Ti，微弧氧化膜层主要由氧化钛、钛酸钙、磷酸钙及偏磷酸钙构成。电镜观察显示1 h 微弧氧化涂层表面细胞已伸出伪足，4 h呈现较典型的细胞形态。细胞在微弧氧化处理钛表面4，7 d的细胞增殖和7，14 d的碱性磷酸酶活性高于对照组。表明微弧氧化技术生成的粗糙多孔钙磷涂层能显著促进MC3T3-E1细胞的早期黏附、增殖及成骨活性。
배경：미호양화기술가개선태혹태합금적표면특정。 　　목적：연구순태표면미호양화도층적표면성능급기대MC3T3-E1세포조기점부、증식급성골능력적영향。 　　방법：장46개직경10 mm、후도2 mm 원반상순태시건분위실험조화대조조。실험조치우함0.02 mol/Lβ-감유린산이납염급0.2 mol/L을산개적전해액중진행미호양화처리，대조조대시건진행궤계포광。소묘전자현미경관찰시건표면형모，X 사선능보분석검측도층표면개린비，X 사선연사분석검측도층정상구성。장MC3T3-E1세포접충재량조시건표면，1，2，4 h전경하관찰세포형태，재2，4，7 d통과CCK-8방법검측세포증식，병우7，14 d검측감성린산매활성。 　　결과여결론：경미호양화처리후，태표면형성조조다공적개린도층，미호양화도층주요원소위Ca、P、O급Ti，미호양화막층주요유양화태、태산개、린산개급편린산개구성。전경관찰현시1 h 미호양화도층표면세포이신출위족，4 h정현교전형적세포형태。세포재미호양화처리태표면4，7 d적세포증식화7，14 d적감성린산매활성고우대조조。표명미호양화기술생성적조조다공개린도층능현저촉진MC3T3-E1세포적조기점부、증식급성골활성。
BACKGROUND:Micro-arc oxidation technique is used to modify the surface properties of titanium and titanium al oy. OBJECTIVE:To explore the surface properties of micro-arc oxidation film and its effect on the attachment, proliferation and alkaline phosphatase activity of MC3T3-E1. METHODS:Forty-six pure titanium discs, 10 mm in diameter and 2 mm in thickness, were randomly divided into two groups. The discs of the experimental groups were treated by micro-arc oxidation technique in an electrolytic solution containing 0.02 mol/L sodiumβ-glycerophosphate and 0.2 mol/L calcium acetate;while the discs of the control group was machine-polished. The surface appearance of the discs was observed by a scanning electron microscopy, the ratio of calcium to phosphorus on the coating surface was detected by X-ray spectroscopy, and the crystal ine phase composition of the coating was detected by X-ray diffraction analysis. cellular morphology in the process of attachment was observed under the scanning electron microscope. cellproliferation was determined by cellcounting kit-8 at 2, 4, 74 days, while alkaline phosphatase activity were determined at 7 and 14 days. RESULTS AND CONCLUSION:After micro-arc oxidation treatment, a rough and porous calcium-phosphate film was formed on the surface of titanium. The elements of micro-arc oxidation coating main mainly included Ca, P, O and Ti, and the micro-arc oxidation film was mainly composed of titanium oxide, calcium titanate, calcium phosphate and calcium metaphosphate. Under the scanning electron microscope, pseudopods appeared to grow out of the cells on the surface of micro-arc oxidation coating after 1 hour culture, and the typical morphology of the MC3T3-E1 cells could be observed at 4 hours. MC3T3-E1 proliferation (4 and 7 days of culture) and alkaline phosphatase activity (7 and 14 days of culture) were enhanced significantly in the micro-arc oxidation group compared with the control group. These findings indicate that the rough and porous calcium-phosphate coating produced by micro-arc oxidation technique on pure titanium surface could promote the early attachment, proliferation and osteogenic differentiation of MC3T3-E1.