CAJ | 학술논문

背景：多项体内外实验表明外源性植入碱性成纤维细胞生长因子能明显促进骨形成过程，但外源性碱性成纤维细胞生长因子在体内易降解，影响疗效。目的：利用分子生物学技术将碱性成纤维细胞生长因子转染至骨髓间充质干细胞中，观察同种异体骨复合基因转染骨髓间充质干细胞修复绵羊极限骨缺损的效果。方法：将同种异体骨复合碱性成纤维细胞生长因子转染骨髓间充质干细胞组织工程骨、骨髓间充质干细胞复合同种异体支架骨材料、同种异体支架骨材料、β-磷酸三钙材料分别植入羊髂骨极限缺损处，植入后4，8，12周行组织学、免疫组织化学染色观察。结果与结论：同种异体骨复合碱性成纤维细胞生长因子转染骨髓间充质干细胞组织工程骨植入后12周，手术结合区成软骨样结构较多，术区中央可见大量成骨样细胞，整个术区的支架材料降解较其他组多，支架材料孔洞内爬满纤维结缔组织，材料周围常见破骨样细胞；骨涎蛋白与Ⅰ型胶原呈强阳性表达。其他3组手术结合区虽有成软骨样结构及成骨样细胞出现，但中央区为死骨结构，且骨涎蛋白与Ⅰ型胶原呈弱表达。表明碱性成纤维细胞生长因子转染的骨髓间充质干细胞复合同种异体骨可基本修复绵羊极限骨缺损。
배경：다항체내외실험표명외원성식입감성성섬유세포생장인자능명현촉진골형성과정，단외원성감성성섬유세포생장인자재체내역강해，영향료효。목적：이용분자생물학기술장감성성섬유세포생장인자전염지골수간충질간세포중，관찰동충이체골복합기인전염골수간충질간세포수복면양겁한골결손적효과。방법：장동충이체골복합감성성섬유세포생장인자전염골수간충질간세포조직공정골、골수간충질간세포복합동충이체지가골재료、동충이체지가골재료、β-린산삼개재료분별식입양가골겁한결손처，식입후4，8，12주행조직학、면역조직화학염색관찰。결과여결론：동충이체골복합감성성섬유세포생장인자전염골수간충질간세포조직공정골식입후12주，수술결합구성연골양결구교다，술구중앙가견대량성골양세포，정개술구적지가재료강해교기타조다，지가재료공동내파만섬유결체조직，재료주위상견파골양세포；골연단백여Ⅰ형효원정강양성표체。기타3조수술결합구수유성연골양결구급성골양세포출현，단중앙구위사골결구，차골연단백여Ⅰ형효원정약표체。표명감성성섬유세포생장인자전염적골수간충질간세포복합동충이체골가기본수복면양겁한골결손。
BACKGROUND:Many in vivo and in vitro experiments indicate that implantation of exogenous basic fibroblast growth factor can significantly promote the process of bone formation, but the in vivo degradation of exogenous basic fibroblast growth factor affects the therapeutic efficacy. <br> OBJECTIVE:To observe the effects of basic fibroblast growth factor-transfected mesenchymal stem cells which transfected using molecular biology techniques combined with al ogeneic bone in the repair of critical-size bone defects in sheep. <br> METHODS:Al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells, bone marrow mesenchymal stem cells combined with al ogeneic bone material stents, al ograft bone material,β-tricalcium calcium material were respectively implanted into critical-size bone defects in sheep. After 4, 8 and 12 weeks, histological and immunohistochemical staining was performed. <br> RESULTS AND CONCLUSION:At 12 weeks after implantation of al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells as tissue engineering bone, there were many cartilage-like structures in the operative binding region and a large amount of osteoblast-like cells in the center of operative region, and there was more material degradation in the entire operative area as compared with other groups;there were fibrous connective tissues ful of the pores, and osteoclast-like cells were commonly seen around the implant material;bone sialoprotein and col agen type Ⅰ expression were strongly positive. In the other three groups, although the cartilage-like structure appeared in the binding region, dead bone structure was found in the central area, and bone sialoprotein and type Ⅰ col agen expression was weak. These findings indicate that al ogeneic bone with basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can basical y repair critical-size bone defects in sheep.