CAJ | 학술논문

背景：细胞支架复合物在体外构建及成熟分化为骨组织工程骨是骨组织工程构建的必须过程，目前尚无统一方法及标准。 　　目的：总结目前骨组织工程构建的技术与基本方法并讨论相关发展。 　　方法：以“bone tissue engineering，cel biological scaffold，cel inoculation，seeding density，culture in vitro， bioreactor”为英文检索词，以“骨组织工程，生物支架，细胞接种，接种密度，体外培养，生物反应器”为中文检索词，检索PubMed数据库和中国期刊全文数据库1997年1月至2013年1月与骨组织工程骨体外构建和外培养相关文献，根据纳入排除标准，最终纳入44篇。 　　结果与结论：生物支架作为骨组织工程种子细胞的载体，首要的前提是无菌，无菌状态的细胞支架复合物才能够存活。生物支架材料的灭菌有紫外线灭菌法，60Coγ射线灭菌法，体积分数75%乙醇浸泡，高压灭菌锅法，环氧乙烷灭菌等方法。60Co γ射线灭菌方法常在支架灭菌中成为较常选的方法。种子细胞的接种密度是影响种子细胞在支架上黏附生长及增殖的一个重要因素。细胞与支架材料的黏附受支架材料亲合力、细胞黏附力及重力等多方面因素的影响。目前阶段骨组织工程种子细胞接种方法分位静态接种和动态接种。每一种构建方法都各有优缺点，克服这些缺点以及形成统一的构建方法并充分应用临床是未来的发展方向。
배경：세포지가복합물재체외구건급성숙분화위골조직공정골시골조직공정구건적필수과정，목전상무통일방법급표준。 　　목적：총결목전골조직공정구건적기술여기본방법병토론상관발전。 　　방법：이“bone tissue engineering，cel biological scaffold，cel inoculation，seeding density，culture in vitro， bioreactor”위영문검색사，이“골조직공정，생물지가，세포접충，접충밀도，체외배양，생물반응기”위중문검색사，검색PubMed수거고화중국기간전문수거고1997년1월지2013년1월여골조직공정골체외구건화외배양상관문헌，근거납입배제표준，최종납입44편。 　　결과여결론：생물지가작위골조직공정충자세포적재체，수요적전제시무균，무균상태적세포지가복합물재능구존활。생물지가재료적멸균유자외선멸균법，60Coγ사선멸균법，체적분수75%을순침포，고압멸균과법，배양을완멸균등방법。60Co γ사선멸균방법상재지가멸균중성위교상선적방법。충자세포적접충밀도시영향충자세포재지가상점부생장급증식적일개중요인소。세포여지가재료적점부수지가재료친합력、세포점부력급중력등다방면인소적영향。목전계단골조직공정충자세포접충방법분위정태접충화동태접충。매일충구건방법도각유우결점，극복저사결점이급형성통일적구건방법병충분응용림상시미래적발전방향。
BACKGROUND:The in vitro construction, maturation and differentiation of cellscaffold complexes into tissue-engineered bone is the necessary process of bone tissue engineering construction, but there are no uniform methods and standards. OBJECTIVE:To summarize the basic method and technology to build bone tissue engineering at present and to discuss the related development. METHODS:A compute-based online search was conducted on the PubMed database and CNKI database for the articles related to the in vitro construction and culture of bone tissue engineering bone from January 1997 to January 2013 with the key words of“bone tissue engineering, cellbiological scaffold, cellinoculation, seeding density, culture in vitro, bioreactor”in English and Chinese. Final y, 44 articles were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:As the carrier of bone tissue engineering seed cells, the primary prerequisite of biological scaffold is sterile, because the sterile biological scaffold can be able to survive. Sterilization of biological scaffolds includes ultraviolet sterilization, 60Coγ-ray sterilization, soaking in ethanol with the volume fraction of 75%, autoclave method, and ethylene oxide sterilization. 60Coγ-ray sterilization is the common method in the biological scaffold sterilization. The inoculation density of seed cells is the key factors that influence the adhesion growth and proliferation of seed cells on the scaffolds. The adhesion between cells and scaffold materials wil be affected by the affinity of scaffolds, celladhesion and gravity, and other factors. The method for the inoculation of bone tissue engineering seed cells includes static inoculation and dynamic inoculation. Each construction method has its advantages and disadvantages. Overcomeing these disadvantages, forming a uniform construction method and ful y clinical application are the direction of future development.