CAJ | 학술논문

背景：单一的血液学分析仅能针对辐射损伤后某个时间点机体的损伤情况，并不能反映机体辐射损伤后的较长期的累积状况。 　　目的：采用基于核磁共振氢谱的代谢组学研究方法确定X射线对小鼠的辐射损伤后尿液和血液中辐射损伤标志物。 　　方法：取小鼠48只随机分为4组，分别接受0(假辐射)，3，9，27 Gy X射线头部辐射，分别于辐射后24 h和5 d后采血检测。另取小鼠36只随机分为3组，分别接受0(假辐射)，9，27 Gy X射线头部辐射，分别于辐射前2 d和辐射后第5天收集24 h尿液检测。采集的血液及尿液进行1H-NMR分析。 　　结果与结论：接受不同剂量X射线辐射后，血清中天冬氨氨基转移酶和丙氨酸氨基转移酶含量没有显著变化，碱性磷酸酶在9 Gy X射线辐射后有显著性升高，表明低剂量头部辐射可能引起肝脏或者骨骼中辐射损伤修复增加；血清中总超氧化物歧化酶在辐射后第5天出现显著降低，表明小鼠经头部辐射后引起了全身氧化应激效应。尿液中N-乙酰甘氨酸和-胸苷在接受各剂量X射线辐射后均显著升高，可以作为头部辐射后尿液中的辐射损伤标志物。尿液中3-羟基-2-甲基苯甲酸-3-氧硫酸盐在接受9 Gy X射线辐射后升高了2.5倍，可以作为中低剂量辐射损伤的特异标志物；尿液牛磺酸在27 Gy X射线辐射后升高20%，可以作为高剂量辐射损伤的特异标志物。
배경：단일적혈액학분석부능침대복사손상후모개시간점궤체적손상정황，병불능반영궤체복사손상후적교장기적루적상황。 　　목적：채용기우핵자공진경보적대사조학연구방법학정X사선대소서적복사손상후뇨액화혈액중복사손상표지물。 　　방법：취소서48지수궤분위4조，분별접수0(가복사)，3，9，27 Gy X사선두부복사，분별우복사후24 h화5 d후채혈검측。령취소서36지수궤분위3조，분별접수0(가복사)，9，27 Gy X사선두부복사，분별우복사전2 d화복사후제5천수집24 h뇨액검측。채집적혈액급뇨액진행1H-NMR분석。 　　결과여결론：접수불동제량X사선복사후，혈청중천동안안기전이매화병안산안기전이매함량몰유현저변화，감성린산매재9 Gy X사선복사후유현저성승고，표명저제량두부복사가능인기간장혹자골격중복사손상수복증가；혈청중총초양화물기화매재복사후제5천출현현저강저，표명소서경두부복사후인기료전신양화응격효응。뇨액중N-을선감안산화-흉감재접수각제량X사선복사후균현저승고，가이작위두부복사후뇨액중적복사손상표지물。뇨액중3-간기-2-갑기분갑산-3-양류산염재접수9 Gy X사선복사후승고료2.5배，가이작위중저제량복사손상적특이표지물；뇨액우광산재27 Gy X사선복사후승고20%，가이작위고제량복사손상적특이표지물。
BACKGROUND:Single hematology analysis can only reflect the body injury at a certain time point after radiation damage, but cannot reflect the longer-term cumulative status after radiation damage. OBJECTIVE:To identify the biomarkers in blood and urine in mice after radiation damage with metabolomics method based on nuclear magnetic resonance hydrogen spectrum. METHODS:Forty-eight mice were randomly divided into four groups and received 0 (sham radiation), 3, 9 and 27 Gy radiation. The blood samples were col ected at 24 hours and 5 days after radiation. Another 36 mice were col ected and divided into three groups and received 0 (sham radiation), 9 and 27 Gy radiation, then the urine samples were col ected at 2 days before radiation and 5 days after radiation for 24 hours. The blood and urine samples were analyzed with proton nuclear magnetic resonance spectroscopy. RESULTS AND CONCLUSION:The content of aspartic aminotransferase and alanine aminotransferase in blood plasma maintained a stable level after 3-or 9-Gy X-ray radiation, but the level of alkaline phosphatase in blood plasma was increased significantly after 9-Gy radiation, which indicating that low-dose head radiation may cause increased radiation damage and repair. The level of total superoxide dismutase in blood plasma was significantly decreased at 5 days after radiation which indicating that head radiation in mice could cause systemic oxidative stress. Meanwhile, the N-hexamolglycine and-thymidine level in the urine samples was significantly increased after different doses X-ray radiation, which can be used as the radiation damage markers in urine samples after head radiation. The 3-hydroxy-2-methylbenzoic acid 3-O-sulfate level in urine samples was increased by 2.5 times after received 9-Gy radiation, which can be used as the specific markers of middle-and low-dose radiation damage;the level of taurine in the urine samples was increased by 20%after 27-Gy radiation, which can be used as the specific markers of high-dose radiation damage.