中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
46期
8043-8048
,共6页
组织构建%组织构建实验造模%兔眼%急性高眼压模型%连通器%Logistic函数%造模时间%眼球进水量%国家自然科学基金
組織構建%組織構建實驗造模%兔眼%急性高眼壓模型%連通器%Logistic函數%造模時間%眼毬進水量%國傢自然科學基金
조직구건%조직구건실험조모%토안%급성고안압모형%련통기%Logistic함수%조모시간%안구진수량%국가자연과학기금
背景：急性高眼压动物模型为研究青光眼提供了简单有效的途径，模型成功与否直接影响研究结果，目前对造模成功的判据多为观察性指标。目的：为前房穿刺所致的急性高眼压动物模型提供准确、可行的造模成功判定方法，观察特定高眼压下急性高眼压动物模型的成模时间。方法：利用连通器原理，将加压装置与内充2 mm 水银球的玻璃管相连，通过读取水银球的流动，计算眼内液体流入量。采用前房灌注法，成功建立10只兔眼眼内压为4.9 kPa 的急性高眼压动物模型，监测眼内液体流入量随造模时间的变化至液体不再流动30 min 后结束实验。拟合眼内压液体流入量随造模时间的变化规律，计算急性高眼压动物模型造模成功的时间。结果与结论：兔眼眼球进水量(y)与加压时间(t)呈Logistic 变化规律，即，其中参数U，b0和b1分别为957.44±313.22，0.023±0.008和0.980±0.003。当注水时间达到(282.93±42.68) min 后，眼内压等于外界施加的高压，兔急性高眼压模型造模成功。
揹景：急性高眼壓動物模型為研究青光眼提供瞭簡單有效的途徑，模型成功與否直接影響研究結果，目前對造模成功的判據多為觀察性指標。目的：為前房穿刺所緻的急性高眼壓動物模型提供準確、可行的造模成功判定方法，觀察特定高眼壓下急性高眼壓動物模型的成模時間。方法：利用連通器原理，將加壓裝置與內充2 mm 水銀毬的玻璃管相連，通過讀取水銀毬的流動，計算眼內液體流入量。採用前房灌註法，成功建立10隻兔眼眼內壓為4.9 kPa 的急性高眼壓動物模型，鑑測眼內液體流入量隨造模時間的變化至液體不再流動30 min 後結束實驗。擬閤眼內壓液體流入量隨造模時間的變化規律，計算急性高眼壓動物模型造模成功的時間。結果與結論：兔眼眼毬進水量(y)與加壓時間(t)呈Logistic 變化規律，即，其中參數U，b0和b1分彆為957.44±313.22，0.023±0.008和0.980±0.003。噹註水時間達到(282.93±42.68) min 後，眼內壓等于外界施加的高壓，兔急性高眼壓模型造模成功。
배경：급성고안압동물모형위연구청광안제공료간단유효적도경，모형성공여부직접영향연구결과，목전대조모성공적판거다위관찰성지표。목적：위전방천자소치적급성고안압동물모형제공준학、가행적조모성공판정방법，관찰특정고안압하급성고안압동물모형적성모시간。방법：이용련통기원리，장가압장치여내충2 mm 수은구적파리관상련，통과독취수은구적류동，계산안내액체류입량。채용전방관주법，성공건립10지토안안내압위4.9 kPa 적급성고안압동물모형，감측안내액체류입량수조모시간적변화지액체불재류동30 min 후결속실험。의합안내압액체류입량수조모시간적변화규률，계산급성고안압동물모형조모성공적시간。결과여결론：토안안구진수량(y)여가압시간(t)정Logistic 변화규률，즉，기중삼수U，b0화b1분별위957.44±313.22，0.023±0.008화0.980±0.003。당주수시간체도(282.93±42.68) min 후，안내압등우외계시가적고압，토급성고안압모형조모성공。
BACKGROUND: An animal model of acute high intraocular pressure offers an easy and effective method to glaucoma research. Research results will be distorted if the animal model is not induced successfully. Nowadays, a successful animal model can only be identified by observational indexes.
<br> OBJECTIVE: To observe the induction time for an animal model of acute high intraocular pressure, and thereby to provide accurate and feasible method for identifying whether the animal model is induced successfully.
<br> METHODS: Acute high intraocular pressure animal models were induced by anterior chamber perfusion using communicating vessels-based device with a glass vessel filled with normal saline and 2-mm-long mercury. Liquid volume was perfused to rabbit eyes. A total of 10 animal models were prepared successfully at an intraocular pressure of 4.9 kPa. The experiment was complete till 30 minutes after the perfused liquid was not moved. At the certain intraocular pressure, the variation of fluid inflow over time was recorded, and the induction time for successful modeling was calculated.
<br> RESULTS AND CONCLUSION: The curve between fluid inflow into rabbit eyes (y) and induction time (t) could be fitted to logistic function, while U, b0 and b1 are 957.44±313.22, 0.023±0.008 and 0.980±0.003, respectively. The fluid inflow to the eye ball did not change when the induction time was over (282.93±42.68) minutes, and the animal model of acute high intraocular pressure was established successfully.
