CAJ | 학술논문

背景：尾侧型同源转录因子2在消化道尤其是小肠与结肠上皮的发育中起到关键作用。 　　目的：构建尾侧型同源转录因子2反转录病毒表达载体pLNCX2-Cdx2，观察pLNCX2-Cdx2体外转染对人膀胱上皮细胞发生肠上皮化生的作用。 　　方法：应用基因重组技术将人尾侧型同源转录因子2cDNA 克隆到反转录病毒载体 pLXSN2,酶切鉴定，包装到PA317细胞中。转染人膀胱尿路上皮细胞，应用荧光PCR及Western blot检测尾侧型同源转录因子2以及绒毛蛋白、LI-cadherin的表达情况。 　　结果与结论：pLNCX2-Cdx2成功构建；细胞转染后尾侧型同源转录因子2的mRNA及蛋白水平表达明显增加；尾侧型同源转录因子2的过表达明显激活了绒毛蛋白与LI-cadherin的表达；转染后细胞可出现肠细胞样特征改变。结果提示体外尾侧型同源转录因子2过表达可使膀胱上皮细胞发生尾侧型同源转录因子2激活并产生肠上皮分化，从而诱导腺性膀胱炎发生。
배경：미측형동원전록인자2재소화도우기시소장여결장상피적발육중기도관건작용。 　　목적：구건미측형동원전록인자2반전록병독표체재체pLNCX2-Cdx2，관찰pLNCX2-Cdx2체외전염대인방광상피세포발생장상피화생적작용。 　　방법：응용기인중조기술장인미측형동원전록인자2cDNA 극륭도반전록병독재체 pLXSN2,매절감정，포장도PA317세포중。전염인방광뇨로상피세포，응용형광PCR급Western blot검측미측형동원전록인자2이급융모단백、LI-cadherin적표체정황。 　　결과여결론：pLNCX2-Cdx2성공구건；세포전염후미측형동원전록인자2적mRNA급단백수평표체명현증가；미측형동원전록인자2적과표체명현격활료융모단백여LI-cadherin적표체；전염후세포가출현장세포양특정개변。결과제시체외미측형동원전록인자2과표체가사방광상피세포발생미측형동원전록인자2격활병산생장상피분화，종이유도선성방광염발생。
BACKGROUND:Caudal type homeobox transcription factor 2 plays an important role in the development of epithelium in digestive tract, especial y the smal intestine and colon. OBJECTIVE:To construct the retroviral expression vector of pLNCX2-caudal type homeobox transcription factor 2, and to observe the effect of in vitro transfection of pLNCX2-caudal type homeobox transcription factor 2 in intestinal metaplasia. METHODS:Gene recombinant technology was employed to clone human caudal type homeobox transcription factor 2 gene to the retroviral expression vector of pLNCX2, then identified with enzyme digestion and sequencing and packed to the PA317 cells. The plasmids were transfect in urothelium cells, real-time PCR and western blot were used to detect the expressions of caudal type homeobox transcription factor 2, vil in and liverintestin-cadherin at the protein and mRNA levels. RESULTS AND CONCLUSION:The retroviral vector pLNCX2-caudal type homeobox transcription factor 2 was successful y constructed. The levels of caudal type homeobox transcription factor 2 protein and mRNA expressions in bladder urothelium celltransfected with pLNCX2-caudal type homeobox transcription factor 2 were higher than that in control. And the over-expression of caudal type homeobox transcription factor 2 could up-regulate the levels of vil in and liverintestin-cadherin expressions. Interestingly, specific changes of intestine-like cells were seen in the bladder urothelium cells transfected by pLNCX2-caudal type homeobox transcription factor 2. Over-expression of caudal type homeobox transcription factor 2 can activate the caudal type homeobox transcription factor 2 of urothelium cells and induce intestinal epithelial differentiation, thus inducing the development of cystitis glanduaris.