中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
46期
8018-8023
,共6页
张玉强%李游%曹阳%董明岩%吕刚
張玉彊%李遊%曹暘%董明巖%呂剛
장옥강%리유%조양%동명암%려강
组织构建%组织构建与生物活性因子%高压氧预处理%高压氧%脊髓损伤%Bcl-2%Bax%免疫组化%脊髓
組織構建%組織構建與生物活性因子%高壓氧預處理%高壓氧%脊髓損傷%Bcl-2%Bax%免疫組化%脊髓
조직구건%조직구건여생물활성인자%고압양예처리%고압양%척수손상%Bcl-2%Bax%면역조화%척수
背景:研究表明高压氧治疗可通过减少脊髓损伤后神经细胞凋亡,促进脊髓神经功能的恢复,但其抑制凋亡的机制还不清楚。<br> 目的:探索高压氧预处理对脊髓损伤后Bcl-2和Bax表达的影响及意义。<br> 方法:将66只健康成年雄性SD大鼠随机分为假手术组6只、模型组和高压氧预处理组各30只。假手术组仅行椎板切除;模型组根据Al en’s打击原理制作脊髓损伤模型,不做任何治疗;高压氧预处理组在10 d内对大鼠进行5次,每次1 h的高压氧(0.25 MPa,纯氧)治疗后,再建立脊髓损伤模型。<br> 结果与结论:免疫组化检测显示假手术组大鼠脊髓组织中Bcl-2和Bax表达较少;模型组和高压氧预处理组大鼠脊髓组织中均可见不同程度的Bcl-2和Bax阳性表达,其中Bcl-2表达高峰出现在损伤后第7天,而Bax表达高峰出现在损伤后第3天;而经高压氧预处理的大鼠脊髓组织中Bcl-2表达增加(P<0.05),而Bax表达减少(P<0.05)。提示高压氧预处理能够增强Bcl-2的表达同时降低Bax的表达。
揹景:研究錶明高壓氧治療可通過減少脊髓損傷後神經細胞凋亡,促進脊髓神經功能的恢複,但其抑製凋亡的機製還不清楚。<br> 目的:探索高壓氧預處理對脊髓損傷後Bcl-2和Bax錶達的影響及意義。<br> 方法:將66隻健康成年雄性SD大鼠隨機分為假手術組6隻、模型組和高壓氧預處理組各30隻。假手術組僅行椎闆切除;模型組根據Al en’s打擊原理製作脊髓損傷模型,不做任何治療;高壓氧預處理組在10 d內對大鼠進行5次,每次1 h的高壓氧(0.25 MPa,純氧)治療後,再建立脊髓損傷模型。<br> 結果與結論:免疫組化檢測顯示假手術組大鼠脊髓組織中Bcl-2和Bax錶達較少;模型組和高壓氧預處理組大鼠脊髓組織中均可見不同程度的Bcl-2和Bax暘性錶達,其中Bcl-2錶達高峰齣現在損傷後第7天,而Bax錶達高峰齣現在損傷後第3天;而經高壓氧預處理的大鼠脊髓組織中Bcl-2錶達增加(P<0.05),而Bax錶達減少(P<0.05)。提示高壓氧預處理能夠增彊Bcl-2的錶達同時降低Bax的錶達。
배경:연구표명고압양치료가통과감소척수손상후신경세포조망,촉진척수신경공능적회복,단기억제조망적궤제환불청초。<br> 목적:탐색고압양예처리대척수손상후Bcl-2화Bax표체적영향급의의。<br> 방법:장66지건강성년웅성SD대서수궤분위가수술조6지、모형조화고압양예처리조각30지。가수술조부행추판절제;모형조근거Al en’s타격원리제작척수손상모형,불주임하치료;고압양예처리조재10 d내대대서진행5차,매차1 h적고압양(0.25 MPa,순양)치료후,재건립척수손상모형。<br> 결과여결론:면역조화검측현시가수술조대서척수조직중Bcl-2화Bax표체교소;모형조화고압양예처리조대서척수조직중균가견불동정도적Bcl-2화Bax양성표체,기중Bcl-2표체고봉출현재손상후제7천,이Bax표체고봉출현재손상후제3천;이경고압양예처리적대서척수조직중Bcl-2표체증가(P<0.05),이Bax표체감소(P<0.05)。제시고압양예처리능구증강Bcl-2적표체동시강저Bax적표체。
BACKGROUND:Studies have shown that hyperbaric oxygen preconditioning can reduce neuronal apoptosis <br> and improve neurologic dysfunction after spinal cord injury. However, the mechanism of inhibiting apoptosis is stil unclear. <br> OBJECTIVE:To study the effect and significance of hyperbaric oxygen preconditioning on expression of Bcl-2 and Bax protein after spinal cord injury in rats. <br> METHODS:Sixty-six male adult Sprague-Dawley rats were divided into sham operation group, model group, treatment group at random. Animals in the sham group were six, and animals in the model and treatment groups were 30, respectively. Animals in the treatment group received hyperbaric oxygen preconditioning at 2.5 MPa (100%O 2 ), once for 1 hour and total y five times within 10 days before establishment of spinal cord injury models. In the sham operation group, only laminectomy was done. In the model group, a spinal cord injury model was prepared using Al en’s method. RESULTS AND CONCLUSION:The results of immunochemistry indicated that there were few Bcl-2 and <br> Bax-positive cells in the sham operation group;a number of Bcl-2 and Bax-positive cells were visible in the model and treatment groups. Bcl-2 and Bax expressions reached the peak at day 7 and 3, respectively, after injury. After treatment with hyperbaric oxygen preconditioning, Bcl-2 positive cells decreased significantly, while Bax-positive cells decreased significantly (P<0.05). It indicates that the hyperbaric oxygen preconditioning can increase Bcl-2 expression but decrease Bax expression.