CAJ | 학술논문

背景：有动物建模方面的报道称心理应激可引起大鼠颞下颌关节疾病的发生，并有病理改变。 　　目的：观察心理应激对大鼠颞下颌关节髁突软骨细胞血管内皮生长因子和转化生长因子β1表达的影响。 　　方法：将48只成年Wistar雄性大鼠随机分为实验组和对照组，其中实验组36只，对照组12只。对实验组施加电击、制动、气候箱、食物诱惑等刺激，使实验组大鼠处于心理应激状态。将实验组分别于刺激3周(刺激3周组)、刺激6周(刺激6周组)和刺激6周后正常饲养6周(恢复组)时间点各处死12只；对照组12只同实验组最后一批一起处死，取颞下颌关节标本制作切片。 　　结果与结论：恢复组血管内皮生长因子阳性率明显大于刺激3周组和对照组(P<0.05)；刺激6周组转化生长因子β1阳性率明显大于对照组(P <0.05)。表明血管内皮生长因子与新生毛细血管修复组织有关，转化生长因子β1在心理应激过程中可能起到了促进骨吸收的作用。
배경：유동물건모방면적보도칭심리응격가인기대서섭하합관절질병적발생，병유병리개변。 　　목적：관찰심리응격대대서섭하합관절과돌연골세포혈관내피생장인자화전화생장인자β1표체적영향。 　　방법：장48지성년Wistar웅성대서수궤분위실험조화대조조，기중실험조36지，대조조12지。대실험조시가전격、제동、기후상、식물유혹등자격，사실험조대서처우심리응격상태。장실험조분별우자격3주(자격3주조)、자격6주(자격6주조)화자격6주후정상사양6주(회복조)시간점각처사12지；대조조12지동실험조최후일비일기처사，취섭하합관절표본제작절편。 　　결과여결론：회복조혈관내피생장인자양성솔명현대우자격3주조화대조조(P<0.05)；자격6주조전화생장인자β1양성솔명현대우대조조(P <0.05)。표명혈관내피생장인자여신생모세혈관수복조직유관，전화생장인자β1재심리응격과정중가능기도료촉진골흡수적작용。
BACKGROUND:Animal modeling has reported that psychological stress can lead temporomandibular joint disease in rats, and there wil be pathological changes. OBJECTIVE:To observe the effects of psychological stress on the expressions of vascular endothelial growth factor and transforming growth factor-β1 in rat temporomandibular joint condylar chondrocytes METHODS:Forty-eight adult male Wistar rats were randomly divided into the experimental group and the control group, 6 rats in the experimental group and 12 rats in the control group. Thirty-six Wister male adult rats in the experimental group were subjected to the fol owing stimulating factors, such as electroshock, keeping stil ness, climate change and food tempting in order to make them under psychological stress. Then, the experimental group rats were executed at 3 weeks after stimulation (3 weeks stimulation group), 6 weeks after stimulation (6 weeks stimulation group) and 6 weeks normal feeding after 6 week stimulation (recovery group), 12 rats were sacrificed at each time point. The 12 rats in the control group were executed at the same time as the last experimental group. The temporomandibular joint specimens were taken to produce slices. RESULTS AND CONCLUSION:The vascular endothelial growth factor-positive rate of recovery group was significantly larger than that of the 3 weeks stimulation group and the control group (P<0.05);transforming growth factor-β1 positive rate in the 6 weeks stimulation group was significantly greater than that in the control group (P<0.05). It indicated that vascular endothelial growth factor was related with the new blood capil aries repairing tissue, and transforming growth factor-β1 might play a role that stimulates bone resorption in psychological stress.