CAJ | 학술논문

采用溴化钾压片法及傅里叶变换红外光谱技术对中药三七（Panax notoginseng）的剪口、主根、筋条、绒根等不同部位以及表皮、韧皮部、形成层和木部等不同组织的红外光谱及其二阶导数谱进行了研究。三七不同部位及组织红外光谱的整体峰形相似；在3400，2930，1645，1155，1080和1020 cm-1附近有淀粉的特征吸收峰，其主体成分均为淀粉。但各部位和组织的红外光谱及其特征性化学成分有差异。剪口在1077和1152 cm-1处峰的强度比大于其他部位，皂苷的含量最高。剪口及其表皮在1317和780 cm -1特征峰明显，含草酸钙；并且剪口表皮中草酸钙的含量比剪口中的含量高。绒根在1384和825 cm -1处有硝酸盐的特征峰，说明绒根富含硝酸盐类成分。主根表皮在2926，2854和1740 cm -1处的吸收峰较强，酯类成分的含量较高。形成层的红外光谱中酰胺Ⅰ带和酰胺Ⅱ带峰明显，富含蛋白质。由此可见，三七不同部位和组织的红外光谱及其二阶导数谱各有特征，不仅揭示了整体化学成分，还能提供有机大分子、无机小分子等特征性成分信息。红外光谱可用于三七及其不同部位和组织的准确、快速鉴别及质量评价。
채용추화갑압편법급부리협변환홍외광보기술대중약삼칠（Panax notoginseng）적전구、주근、근조、융근등불동부위이급표피、인피부、형성층화목부등불동조직적홍외광보급기이계도수보진행료연구。삼칠불동부위급조직홍외광보적정체봉형상사；재3400，2930，1645，1155，1080화1020 cm-1부근유정분적특정흡수봉，기주체성분균위정분。단각부위화조직적홍외광보급기특정성화학성분유차이。전구재1077화1152 cm-1처봉적강도비대우기타부위，조감적함량최고。전구급기표피재1317화780 cm -1특정봉명현，함초산개；병차전구표피중초산개적함량비전구중적함량고。융근재1384화825 cm -1처유초산염적특정봉，설명융근부함초산염류성분。주근표피재2926，2854화1740 cm -1처적흡수봉교강，지류성분적함량교고。형성층적홍외광보중선알Ⅰ대화선알Ⅱ대봉명현，부함단백질。유차가견，삼칠불동부위화조직적홍외광보급기이계도수보각유특정，불부게시료정체화학성분，환능제공유궤대분자、무궤소분자등특정성성분신식。홍외광보가용우삼칠급기불동부위화조직적준학、쾌속감별급질량평개。
The techniques of Fourier transform infrared (FTIR) spectroscopy were applied to analyze the different parts and tis-sues of Panax Notoginseng (Sanqi ,SQ) ,i .e .rhizome ,main root ,rootlet ,fibrous root ,xylem ,cambium ,phloem and epider-mis .Both the FTIR spectra and second derivative spectra of these various parts and tissues of SQ samples were found to be simi-lar .Their dominant component is starch resulting from the characteristic peaks of starch observed at 3 400 ,2 930 ,1 645 , 1 155 ,1 080 and 1 020 cm -1 on the spectra of all these SQ samples .However ,the varieties of peaks were found on the spectra among these specific samples .The rhizome contains more saponins than others on the basis of the largest ratio of the peak inten-sity at 1 077 cm -1 to that at 1 152 cm -1 .The peaks located at 1 317 and 780 cm -1 on the FTIR spectra of the rhizome and its ep-idermis indicate that the two parts of SQ samples contain large amount of calcium oxalate ,and its content in the latter is relative larger than that in former .The fibrous root contains much amount of nitrate owing to the obvious characteristic peaks at 1 384 and 831 cm -1 .For the difference among the various tissues of SQ samples ,the peaks at 2 926 ,2 854 and 1 740 cm-1 on the FT-IR spectra of epidermis is the strongest among the various tissues of main root indicating the largest amount of esters in epider-mis .Protein was also found in the cambium of the main root based on the relative strong peaks of amide Ⅰ and Ⅱ band at 1 641 and 1 541 cm -1 ,respectively .The results indicate that FTIR spectra with its second derivative spectra can show the characteris-tic of the various parts and tissues of SQ samples in both the holistic chemical constituents and specific chemical components ,in-cluding organic macromolecule compounds and small inorganic molecule compounds .FTIR spectroscopy is a useful analytical method for the genuine and rapid identification and quality assessment of SQ samples .