CAJ | 학술논문

使用表面等离子共振谱（surface plasmon resonance ，SPR）检测葡萄糖浓度，引入D-半乳糖/D-葡萄糖结合蛋白（D-galactose/D-glucose binding protein ，GGBP），该蛋白能够选择性吸附葡萄糖分子，实现特异性、高分辨率地检测葡萄糖分子的浓度。研究了在SPR传感器表面利用硫醇耦合绑定蛋白的方法，成功在SPR传感器表面绑定了GGBP蛋白并对不同浓度的葡萄糖进行了测量实验。实验结果显示，当葡萄糖浓度为0.1～10 mg · dL -1时，采用硫醇耦合方法修饰蛋白的传感器具有良好的线性，同时也具有很好的重复性和稳定性，在SPR传感器表面绑定GGBP蛋白满足了组织液中低浓度葡萄糖检测的需求。
사용표면등리자공진보（surface plasmon resonance ，SPR）검측포도당농도，인입D-반유당/D-포도당결합단백（D-galactose/D-glucose binding protein ，GGBP），해단백능구선택성흡부포도당분자，실현특이성、고분변솔지검측포도당분자적농도。연구료재SPR전감기표면이용류순우합방정단백적방법，성공재SPR전감기표면방정료GGBP단백병대불동농도적포도당진행료측량실험。실험결과현시，당포도당농도위0.1～10 mg · dL -1시，채용류순우합방법수식단백적전감기구유량호적선성，동시야구유흔호적중복성화은정성，재SPR전감기표면방정GGBP단백만족료조직액중저농도포도당검측적수구。
In the glucose measuring technique by surface plasmon resonance ,D-galactose/D-glucose binding protein (GGBP) that can specifically adsorb glucose was introduced ,and high-precision specific detection of glucose concentration was realized .In the present paper ,the GGBP protein was bound on the surface of SPR sensor through thiol coupling method .GGBP binding experi-ment was carried out on SPR sensor and then glucose concentration experiment was conducted with this sensor .The results indi-cated that the SPR sensor had good linearity ,stability and repeatability in the range of 0.1~10 mg · dL -1 .SPR sensor bound with GGBP would have great potential and vast development prospects .