现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
17期
1825-1827
,共3页
陈阳%付正英%张引国%彭京丽
陳暘%付正英%張引國%彭京麗
진양%부정영%장인국%팽경려
S100A 11%胚胎植入%子宫内膜容受性
S100A 11%胚胎植入%子宮內膜容受性
S100A 11%배태식입%자궁내막용수성
S100 A11%embryo planting%endometrial receptivity
目的:研究钙结合蛋白S100 A11在小鼠胚胎种植早期子宫内膜中的表达及该蛋白在子宫内膜容受性中的作用。方法采用免疫组织化学法、Real-time PCR和Western blot法分别检测钙结合蛋白S100A11在妊娠早期子宫的细胞定位、mRNA和蛋白表达情况。结果免疫组化结果显示S100 A11蛋白主要分布于子宫内膜腺上皮和腔上皮;Real-time PCR结果表明S100A11mRNA在围着床期均有表达,其中妊娠第4天表达量最高;Western Blot表明S100 A11蛋白在围着床期子宫中均有表达,并在妊娠第4天、第5天明显升高。结论钙结合蛋白S100 A11调节了胚胎在子宫内膜上的黏附与侵入,参与了子宫内膜的容受性及免疫耐受。
目的:研究鈣結閤蛋白S100 A11在小鼠胚胎種植早期子宮內膜中的錶達及該蛋白在子宮內膜容受性中的作用。方法採用免疫組織化學法、Real-time PCR和Western blot法分彆檢測鈣結閤蛋白S100A11在妊娠早期子宮的細胞定位、mRNA和蛋白錶達情況。結果免疫組化結果顯示S100 A11蛋白主要分佈于子宮內膜腺上皮和腔上皮;Real-time PCR結果錶明S100A11mRNA在圍著床期均有錶達,其中妊娠第4天錶達量最高;Western Blot錶明S100 A11蛋白在圍著床期子宮中均有錶達,併在妊娠第4天、第5天明顯升高。結論鈣結閤蛋白S100 A11調節瞭胚胎在子宮內膜上的黏附與侵入,參與瞭子宮內膜的容受性及免疫耐受。
목적:연구개결합단백S100 A11재소서배태충식조기자궁내막중적표체급해단백재자궁내막용수성중적작용。방법채용면역조직화학법、Real-time PCR화Western blot법분별검측개결합단백S100A11재임신조기자궁적세포정위、mRNA화단백표체정황。결과면역조화결과현시S100 A11단백주요분포우자궁내막선상피화강상피;Real-time PCR결과표명S100A11mRNA재위착상기균유표체,기중임신제4천표체량최고;Western Blot표명S100 A11단백재위착상기자궁중균유표체,병재임신제4천、제5천명현승고。결론개결합단백S100 A11조절료배태재자궁내막상적점부여침입,삼여료자궁내막적용수성급면역내수。
Objective It is to research the expression of calcium-binding protein S100A11 in mouse endometrium in early embryo plantation stage and the function of this protein in endometrial receptivity .Methods Real-time PCR was used to detect the concentration of S100A11 mRNA, immunohistochemical and western blot method was used to detect the concentration of S100A11 protein expression in the uterine tissue in peri-implantation.Results The immunohistochemical results showed that S100A11 was mainly located in luminal and glandular epithelium of endometrium .Real-time PCR results indicated that the ex-pression of S100A11 mRNA could be found during peri-implantation, in which the expression was the highest on the fourth day of pregnancy.Western blot results prompted that the expression S 100A11 could be found in the implantation window phase of endometrium , which was much higher on the fourth day and fifth day of pregnancy than that of other days .Conclusion The specific expression of S100A11 gene in endometrium regulates the adhesion and invasion of embryo , and it can be participated in the formation of endometrial capacity and immune tolerance .