CAJ | 학술논문

活性氧（ ROS）作为一种重要信号分子在植物体生命活动的多方面发挥着关键作用。为对活细胞内的ROS分子进行研究分析，人们发展出了多种检测手段。其中，荧光探针作为一种高度灵敏、直观的标记技术，在植物ROS研究中得到广泛应用。近年来，通过蛋白质工程和基因工程技术，研究者们基于绿色荧光蛋白创造出一批新型的特异性荧光探针，为进一步探索植物体内ROS信号途径提供更有力的工具。本文概述了植物细胞中ROS信号转导通路，并总结针对不同ROS组分的多种研究方法，尤其对利用荧光探针标记技术检测胞内ROS的研究进展进行详细介绍。
활성양（ ROS）작위일충중요신호분자재식물체생명활동적다방면발휘착관건작용。위대활세포내적ROS분자진행연구분석，인문발전출료다충검측수단。기중，형광탐침작위일충고도령민、직관적표기기술，재식물ROS연구중득도엄범응용。근년래，통과단백질공정화기인공정기술，연구자문기우록색형광단백창조출일비신형적특이성형광탐침，위진일보탐색식물체내ROS신호도경제공경유력적공구。본문개술료식물세포중ROS신호전도통로，병총결침대불동ROS조분적다충연구방법，우기대이용형광탐침표기기술검측포내ROS적연구진전진행상세개소。
Reactive oxygen species ( ROS) play important roles in plant life. Several ROS detecting methods have been applied in in vivo studies of ROS. Among them, fluorescent probes have been widely employed in plant sciences, since researchers can visualize the cellular distribution and changing of ROS with high sensitivity. Currently, advanced protein and genetic engineering created several novel fluorescent approaches, providing new molecular tools for further exploring of the ROS signaling pathways in live plant cells. This review summarizes the ROS signal transduction pathway and detection methods for various ROS species in plants, especially those based on intracellular fluorescence labeled probes.