临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
4期
372-374
,共3页
脑膜炎%肺炎链球菌%神经干细胞%室管膜下区%大鼠
腦膜炎%肺炎鏈毬菌%神經榦細胞%室管膜下區%大鼠
뇌막염%폐염련구균%신경간세포%실관막하구%대서
meningitis%Streptococcus pneumoniae%neural stem cells%subventricular zone%rat
目的：观察内源性神经干细胞在幼年大鼠肺炎链球菌性脑膜炎室管膜下区的增殖。方法3周龄SD大鼠分为感染组（n=28）、对照组（n=16）和假手术组（n=16）。感染组脑池穿刺接种肺炎链球菌，对照组注射生理盐水，假手术组仅剪开皮肤，分别于接种后24 h，及5、14及21天各处死4只大鼠，采用免疫组化法检测神经干细胞间接标志物5-溴脱氧尿核苷（Brdu）的表达，观察内源性神经干细胞在室管膜下区的增殖情况。结果感染组、对照组及假手术组大鼠在接种后第24小时、5天、14天及21天时，双侧室管膜下区均可见Brdu阳性细胞，感染组的Brdu阳性细胞在接种后24小时开始增加，第5天达到高峰，随后逐渐下降，各时间点的差异有统计学意义（P<0.05）。在接种后24小时、5天和14天时，感染组的Brdu阳性细胞数均高于对照组及假手术组，差异有统计学意义（P<0.05）；接种后第21天时，感染组与对照组的差异无统计学意义（P>0.05），但与假手术组的差异仍有统计学意义（P<0.05）。结论肺炎链球菌性脑膜炎可刺激幼年大鼠室管膜下区神经干细胞的增殖。
目的：觀察內源性神經榦細胞在幼年大鼠肺炎鏈毬菌性腦膜炎室管膜下區的增殖。方法3週齡SD大鼠分為感染組（n=28）、對照組（n=16）和假手術組（n=16）。感染組腦池穿刺接種肺炎鏈毬菌，對照組註射生理鹽水，假手術組僅剪開皮膚，分彆于接種後24 h，及5、14及21天各處死4隻大鼠，採用免疫組化法檢測神經榦細胞間接標誌物5-溴脫氧尿覈苷（Brdu）的錶達，觀察內源性神經榦細胞在室管膜下區的增殖情況。結果感染組、對照組及假手術組大鼠在接種後第24小時、5天、14天及21天時，雙側室管膜下區均可見Brdu暘性細胞，感染組的Brdu暘性細胞在接種後24小時開始增加，第5天達到高峰，隨後逐漸下降，各時間點的差異有統計學意義（P<0.05）。在接種後24小時、5天和14天時，感染組的Brdu暘性細胞數均高于對照組及假手術組，差異有統計學意義（P<0.05）；接種後第21天時，感染組與對照組的差異無統計學意義（P>0.05），但與假手術組的差異仍有統計學意義（P<0.05）。結論肺炎鏈毬菌性腦膜炎可刺激幼年大鼠室管膜下區神經榦細胞的增殖。
목적：관찰내원성신경간세포재유년대서폐염련구균성뇌막염실관막하구적증식。방법3주령SD대서분위감염조（n=28）、대조조（n=16）화가수술조（n=16）。감염조뇌지천자접충폐염련구균，대조조주사생리염수，가수술조부전개피부，분별우접충후24 h，급5、14급21천각처사4지대서，채용면역조화법검측신경간세포간접표지물5-추탈양뇨핵감（Brdu）적표체，관찰내원성신경간세포재실관막하구적증식정황。결과감염조、대조조급가수술조대서재접충후제24소시、5천、14천급21천시，쌍측실관막하구균가견Brdu양성세포，감염조적Brdu양성세포재접충후24소시개시증가，제5천체도고봉，수후축점하강，각시간점적차이유통계학의의（P<0.05）。재접충후24소시、5천화14천시，감염조적Brdu양성세포수균고우대조조급가수술조，차이유통계학의의（P<0.05）；접충후제21천시，감염조여대조조적차이무통계학의의（P>0.05），단여가수술조적차이잉유통계학의의（P<0.05）。결론폐염련구균성뇌막염가자격유년대서실관막하구신경간세포적증식。
Objective To investigate the proliferation of endogenous neural stem cells in rat brain in the course of experi-mental Streptococcus pneumoniae (Sp) meningitis. Methods Three-week-old SD rats were divided into Sp meningitis group (n=28), control group (n=16) and sham operation group (n=16). The rats in Sp meningitis group were inoculated with Sp by cister-nal puncture and rats in control group were injected with normal saline. Four rats each were individually sacrificed at 24 h, 5, 14 and 21 days after inoculation and the expressions of Bromodeoxyuridine (Brdu) in the subventricular zone and hippocampus of the rats were dynamically detected by immunohistochemical method. Results Brdu positive cells were observed in the bilateral subventricular zone of the rats in Sp meningitis group, control group and sham operation group at 24h, 5, 14 and 21 days after in-oculation. The number of Brdu positive cells in Sp meningitis group was increased initially at 24h after inoculation, reaching a peak at 5th day and then decreased. There was difference among different time points (P<0.05). At 24h, 5 and 14 days after inocu-lation, the number of Brdu positive cells was respectively significantly higher in Sp meningitis group than that in control group and sham-operation group (P<0.05). At 21th day after inoculation, the number of Brdu positive cells in Sp meningitis group was similar with control group (P>0.05), while different from sham operation group (P<0.05). Conclusions Sp meningitis can stimu-late the proliferation of endogenous neural stem cells in the subventricular zone of rat brain.
