中国民族民间医药
中國民族民間醫藥
중국민족민간의약
CHINESE JOURNAL OF ETHNOMEDICINE AND ETHNOPHARMACY
2014年
6期
31-31,33
,共2页
血栓通注射液%5-羟甲基糠醛%高效液相色谱法
血栓通註射液%5-羥甲基糠醛%高效液相色譜法
혈전통주사액%5-간갑기강철%고효액상색보법
xueshuantong injection%5-Hydroxylmethyl-furfural%HPLC
目的：建立高效液相色谱法测定血栓通注射液中5-羟甲基糠醛的含量。方法：色谱柱为CAPCELLPAK C18柱(4．6mm ×250mm,5μm),流动相为甲醇-0．1%磷酸溶液(10∶90)；流速为0．8ml/min,柱温25℃,检测波长284nm。结果：5-羟甲基糠醛进样量在0．0677~270．8ng内呈良好的线性关系(r=1．0000),平均回收率为103．07%, RSD为2．8%。结论：本方法简便、灵敏、准确可靠,专属性强。
目的：建立高效液相色譜法測定血栓通註射液中5-羥甲基糠醛的含量。方法：色譜柱為CAPCELLPAK C18柱(4．6mm ×250mm,5μm),流動相為甲醇-0．1%燐痠溶液(10∶90)；流速為0．8ml/min,柱溫25℃,檢測波長284nm。結果：5-羥甲基糠醛進樣量在0．0677~270．8ng內呈良好的線性關繫(r=1．0000),平均迴收率為103．07%, RSD為2．8%。結論：本方法簡便、靈敏、準確可靠,專屬性彊。
목적：건립고효액상색보법측정혈전통주사액중5-간갑기강철적함량。방법：색보주위CAPCELLPAK C18주(4．6mm ×250mm,5μm),류동상위갑순-0．1%린산용액(10∶90)；류속위0．8ml/min,주온25℃,검측파장284nm。결과：5-간갑기강철진양량재0．0677~270．8ng내정량호적선성관계(r=1．0000),평균회수솔위103．07%, RSD위2．8%。결론：본방법간편、령민、준학가고,전속성강。
Objective To establish a HPLC method to determine the content of 5-Hydroxymethyl-furfural in xueshuantong injec-tion. Method The CAPCELLPAK C18 column (4. 6mm × 250mm, 5μm) was used, the mobile phase was methanol -0. 1% Phos-phoric acid solution (10: 90), the flow rate was 0. 8 ml/min, the temperature of column was 25℃, the detection wavelength was 284nm. Results 5-Hydroxymethyl-furfural showed a good linear relationship in a range of 0. 0677~270. 8 ng (r =1. 0000) . The average recovery was 103. 07%, and RSD was 2. 8%. Conclusion The method is simple, sensitive, specific and accurate.
