实用口腔医学杂志
實用口腔醫學雜誌
실용구강의학잡지
JOURNAL OF PRACTICAL STOMATOLOGY
2014年
6期
748-752
,共5页
尹音%陈新%李冬霞%张婧
尹音%陳新%李鼕霞%張婧
윤음%진신%리동하%장청
高正加速度(+Gz)%大鼠%牙髓%热休克蛋白70(HSP70)%丹参多酚酸盐(DSSM)
高正加速度(+Gz)%大鼠%牙髓%熱休剋蛋白70(HSP70)%丹參多酚痠鹽(DSSM)
고정가속도(+Gz)%대서%아수%열휴극단백70(HSP70)%단삼다분산염(DSSM)
+Gz%Rats%Pulp%Heat shock protein(HSP70)%Depside salt from salvia miltiorrhiza(DSSM)
目的:探讨丹参多酚酸盐(DSSM)对高正加速度(+Gz)重复作用下的大鼠磨牙牙髓的作用。方法:27只 SD 雄性大鼠用随机区组法分为9组,每组3只。阳性对照组和药物组分别在+5 Gz、+10 Gz 的条件下共离心30 s,每天重复5次,间隔以+1Gz 持续60 s,4 d /周,共3周。实验期间药物组于每次离心前30 min 按剂量腹腔注射 DSSM溶液(低、中、高给药剂量分别为2、6、18 mg/kg),阴性及阳性对照组注射等体积生理盐水。实验结束后,光镜下观察各组大鼠牙髓组织形态学变化情况,并采用 Real time qPCR 方法测定大鼠磨牙牙髓中热休克蛋白(HSP70)mRNA 相对含量表达的情况。结果:+5 Gz 和+10 Gz阳性对照组大鼠活动程度降低,磨牙牙髓中出现空泡性变,组间差异不明显。HSP70 mRNA 的相对含量比阴性对照组高(P <0.05)。药物组中 HSP70 mRNA 的相对表达量较各自阳性对照组降低,+10 Gz 高剂量组有统计学意义(P <0.05)。结论:+Gz可引起牙髓损伤,+Gz 值的增加与 HSP70 mRNA 相对表达量增加相关,DSSM可能对牙髓有保护作用。
目的:探討丹參多酚痠鹽(DSSM)對高正加速度(+Gz)重複作用下的大鼠磨牙牙髓的作用。方法:27隻 SD 雄性大鼠用隨機區組法分為9組,每組3隻。暘性對照組和藥物組分彆在+5 Gz、+10 Gz 的條件下共離心30 s,每天重複5次,間隔以+1Gz 持續60 s,4 d /週,共3週。實驗期間藥物組于每次離心前30 min 按劑量腹腔註射 DSSM溶液(低、中、高給藥劑量分彆為2、6、18 mg/kg),陰性及暘性對照組註射等體積生理鹽水。實驗結束後,光鏡下觀察各組大鼠牙髓組織形態學變化情況,併採用 Real time qPCR 方法測定大鼠磨牙牙髓中熱休剋蛋白(HSP70)mRNA 相對含量錶達的情況。結果:+5 Gz 和+10 Gz暘性對照組大鼠活動程度降低,磨牙牙髓中齣現空泡性變,組間差異不明顯。HSP70 mRNA 的相對含量比陰性對照組高(P <0.05)。藥物組中 HSP70 mRNA 的相對錶達量較各自暘性對照組降低,+10 Gz 高劑量組有統計學意義(P <0.05)。結論:+Gz可引起牙髓損傷,+Gz 值的增加與 HSP70 mRNA 相對錶達量增加相關,DSSM可能對牙髓有保護作用。
목적:탐토단삼다분산염(DSSM)대고정가속도(+Gz)중복작용하적대서마아아수적작용。방법:27지 SD 웅성대서용수궤구조법분위9조,매조3지。양성대조조화약물조분별재+5 Gz、+10 Gz 적조건하공리심30 s,매천중복5차,간격이+1Gz 지속60 s,4 d /주,공3주。실험기간약물조우매차리심전30 min 안제량복강주사 DSSM용액(저、중、고급약제량분별위2、6、18 mg/kg),음성급양성대조조주사등체적생리염수。실험결속후,광경하관찰각조대서아수조직형태학변화정황,병채용 Real time qPCR 방법측정대서마아아수중열휴극단백(HSP70)mRNA 상대함량표체적정황。결과:+5 Gz 화+10 Gz양성대조조대서활동정도강저,마아아수중출현공포성변,조간차이불명현。HSP70 mRNA 적상대함량비음성대조조고(P <0.05)。약물조중 HSP70 mRNA 적상대표체량교각자양성대조조강저,+10 Gz 고제량조유통계학의의(P <0.05)。결론:+Gz가인기아수손상,+Gz 치적증가여 HSP70 mRNA 상대표체량증가상관,DSSM가능대아수유보호작용。
Objective:To investigate effects of depside salt from salvia miltiorrhiza(DSSM)on the dental pulp of rats under repeated exposure to +Gz acceleration.Methods:27 male SD rats were randomly divided into 9 groups(n =3).The rats in negative control group was only fixed with rats kept-keeling device,in positive control group were exposed to +5 Gz and +10 Gz respectively for 30 s each time,5 times per day,4 days per week,for 3 consecutive weeks.The rats in other groups were respectively given DSSMat 2,6 and 18 mg/kg respectively 30 min before each +Gz exposure.The rats in negative and positive control groups were injected with 0.9%sodium chloride solution 30 min prior to +Gz exposure.After the experiment,The pulp changes were observed by HE staining.Real time qPCR was applied to determine the relative expression of heat sheck protein(HSP70)mRNA.Results:+Gz exposure decreased the activity degree of the rats,and resulted in damage of rat dental pulp.With the increase of +Gz value,the relative expression of HSP70 mRNA was increased(P <0.05).HSP70 mRNA expression in drug groups was less than that in their own positive control groups,but showed statistical significance only in the 18 mg/kg and +10 Gz group(P <0.05).Conclusion:+Gz exposure may dam-age dental pulp.DSSMhas certain protective effect under +Gz exposure.