CAJ | 학술논문

目的：体外研究改良富血小板血浆（modified platelet-rich plasma，mPRP）促进人乳牙牙髓干细胞成骨分化的作用。方法：以α-MEM作为基础培养基，分别加入1％、2％、5％、10％4种不同浓度 mPRP 或者10％胎牛血清（对照），对第4代SHED 连续培养并诱导矿化，碱性磷酸酶试剂盒检测 ALP 活性的变化，qRT-PCR 方法检测细胞内 RUNX2和骨钙素 mRNA 含量的改变。结果：不同浓度的 mPRP 均可以促进乳牙牙髓干细胞的 ALP 活性，且浓度为2％时 A 值最高；qRT-PCR 检测显示2％ mPRP 可以上调乳牙牙髓干细胞内 RUNX2及骨钙素 mRNA 的含量。结论：一定浓度的 mPRP 对乳牙牙髓干细胞的成骨分化具有一定的促进作用。
목적：체외연구개량부혈소판혈장（modified platelet-rich plasma，mPRP）촉진인유아아수간세포성골분화적작용。방법：이α-MEM작위기출배양기，분별가입1％、2％、5％、10％4충불동농도 mPRP 혹자10％태우혈청（대조），대제4대SHED 련속배양병유도광화，감성린산매시제합검측 ALP 활성적변화，qRT-PCR 방법검측세포내 RUNX2화골개소 mRNA 함량적개변。결과：불동농도적 mPRP 균가이촉진유아아수간세포적 ALP 활성，차농도위2％시 A 치최고；qRT-PCR 검측현시2％ mPRP 가이상조유아아수간세포내 RUNX2급골개소 mRNA 적함량。결론：일정농도적 mPRP 대유아아수간세포적성골분화구유일정적촉진작용。
Objective:To investigate the effect of modified plateletrich plasma(mPRP)on the osteogenic differentiation of stemcells from human exfoliated deciduous teeth(SHED).Methods:mPRP at 1%,2%,5%,10% and FBS at 10% were added to thecultured SHED of passage 4,respectively.The influence of mPRP on alkaline phosphatase(ALP)activity was evaluated using ALPkit.RUNX2 and osteocalcin mRNA expression in the treated cells were examined by realtime PCR.Results:mPRP enhanced ALPactivity in the SHED,and the effect of mPRP was more obvious at 2%.Treatment of the cells with 2% mPRP upregulated the mRNAexpressions of RUNX2 and osteocalcin.Conclusion:mPRP can promote the osteogenic differentiation of SHED.