CAJ | 학술논문

目的：探讨慢性牙周炎患者中吸烟个体血清在具核梭杆菌（Fn）侵入 KB 细胞及在侵入过程中对 KB 细胞分泌基质金属蛋白酶-1（MMP-1）的作用。方法：提取慢性牙周炎吸烟个体10例（Y 组）、慢性牙周炎非吸烟个体10例（N 组）、牙周健康非吸烟个体5例（H 组）的血清。分别将各组血清200、400、800μl 添加至 Fn 感染 KB 细胞模型，作用24 h 后计数细菌菌落。用人酶联免疫吸附测定试剂盒检测各组上清液中 MMP-1的浓度。用单因素方差分析进行数据分析。结果：3组800μl血清引起 Fn 侵入 KB 细胞的侵入率（％）分别为12．59±1．27、8．03±0．075和7．99±0．14（P ＜0．05），KB 细胞分泌 MMP-1的浓度（μg/L）分别为400．04±21．02、252．57±18．89、262．47±35．29（P ＜0．05）。200μl 和400μl 血清组与 N 组、H 组比较， Fn 侵入率及 KB 细胞 MMP-1分泌量无统计学差异（P ＞0．05）。结论：较高剂量吸烟个体血清有利于 Fn 侵入 KB 细胞并促进KB 细胞分泌 MMP-1。
목적：탐토만성아주염환자중흡연개체혈청재구핵사간균（Fn）침입 KB 세포급재침입과정중대 KB 세포분비기질금속단백매-1（MMP-1）적작용。방법：제취만성아주염흡연개체10례（Y 조）、만성아주염비흡연개체10례（N 조）、아주건강비흡연개체5례（H 조）적혈청。분별장각조혈청200、400、800μl 첨가지 Fn 감염 KB 세포모형，작용24 h 후계수세균균락。용인매련면역흡부측정시제합검측각조상청액중 MMP-1적농도。용단인소방차분석진행수거분석。결과：3조800μl혈청인기 Fn 침입 KB 세포적침입솔（％）분별위12．59±1．27、8．03±0．075화7．99±0．14（P ＜0．05），KB 세포분비 MMP-1적농도（μg/L）분별위400．04±21．02、252．57±18．89、262．47±35．29（P ＜0．05）。200μl 화400μl 혈청조여 N 조、H 조비교， Fn 침입솔급 KB 세포 MMP-1분비량무통계학차이（P ＞0．05）。결론：교고제량흡연개체혈청유리우 Fn 침입 KB 세포병촉진KB 세포분비 MMP-1。
Objective:To investigate the effects of serum from smoking individuals with periodontitis in the process of Fusobocterium.Nucleatum(Fn)invading KB cells and the expression of matrixmetalloproteinase1(MMP1)of KB cells.Methods:Serum was prepared from 10 smokers with periodontitis(group Y),10 nonsmokers with periodontitis(group N)and 5 periodontal healthy nonsmokers(group H).Serum of 200,400 and 800 μl from the subjects was added to the model of Fn invading KB cells respectively and cultured for 24 hours,the cfu of cellinvaded bacteria was estimated by colony counting.MMP1 protein level in culture supernatant wasmeasured by ELISA.Results:In the 800 μl serum groups,the percentage of invaded cfu was 12.59 ±1.27,8.03 ±0.075 and 7.99±0.14 in group Y,N and H(P <0.05)respectively,the concentration(μg/L)of MMP1 in the cultrue supernatant of group Y,Nand H was 400.04 ±21.02,252.57 ±18.89 and 262.47 ±35.29(P <0.05)respectively.In the 200 μl and 400 μl serum groups,no significant difference was detected in invasion cfu of Fn in KB cells and MMP1 secrition of KB cells among Y,N and H groups(P>0.05).Conclusion:Higher doses of smokingserum might play a role in the course of Fn invading KB cells and promote the expression of MMP1 secretion from KB cells.