CAJ | 학술논문

[目的]探讨金银花水煎剂对D-半乳糖致衰模型小鼠的抗氧化作用。[方法]（1）取一月龄ICR小鼠50只，随机分为对照组、衰老组、金银花高、中、低剂量组。D-半乳糖构建衰老模型过程中，金银花高(10g·kg-1)、中(5g·kg-1)、低(2.5g·kg-1)剂量组每天按剂量分别灌胃。对照组和衰老组灌等量的生理盐水。连续6W后采集小鼠血清样品和各组织匀浆样品进行生化指标SOD、MDA、GSH-Px检测。[结果]42d后，衰老组小鼠出现不同程度的行动缓慢，皮毛光泽暗淡；体重低于正常组；血清、肝、肾、大脑组织中的SOD值、GSH-Px水平低于正常组；血清、肝、肾组织中的MDA含量均高于正常组（P<0.05或P<0.01）；以上数据说明造模基本成功。与衰老组比较，金银花给药组各项指标明显改善，金银花给药组体重增加高于衰老组（P<0.01）；血清、肝、肾SOD值及GSH-Px水平均高于衰老组（P<0.05或P<0.01），MDA含量低于衰老组（P<0.05或P<0.01）。[结论]金银花对D-半乳糖致衰老小鼠具有抗氧化作用，且中剂量组(5g·kg-1)效果显著。
[목적]탐토금은화수전제대D-반유당치쇠모형소서적항양화작용。[방법]（1）취일월령ICR소서50지，수궤분위대조조、쇠로조、금은화고、중、저제량조。D-반유당구건쇠로모형과정중，금은화고(10g·kg-1)、중(5g·kg-1)、저(2.5g·kg-1)제량조매천안제량분별관위。대조조화쇠로조관등량적생리염수。련속6W후채집소서혈청양품화각조직균장양품진행생화지표SOD、MDA、GSH-Px검측。[결과]42d후，쇠로조소서출현불동정도적행동완만，피모광택암담；체중저우정상조；혈청、간、신、대뇌조직중적SOD치、GSH-Px수평저우정상조；혈청、간、신조직중적MDA함량균고우정상조（P<0.05혹P<0.01）；이상수거설명조모기본성공。여쇠로조비교，금은화급약조각항지표명현개선，금은화급약조체중증가고우쇠로조（P<0.01）；혈청、간、신SOD치급GSH-Px수평균고우쇠로조（P<0.05혹P<0.01），MDA함량저우쇠로조（P<0.05혹P<0.01）。[결론]금은화대D-반유당치쇠로소서구유항양화작용，차중제량조(5g·kg-1)효과현저。
[Objective]To study the antioxidant effect of Lonicera japonica extract in D-galactose induced aging model mice. [Methods] Fifty 1-month-old ICR mice were randomly divided into 5 groups: control group, aging group, Lonicera japonica high, medium and low dose groups. During building the aging model, the Lonicera japonica high(10g·kg-1),medium(5g·kg-1) and low(2.5g ·kg-1 ) dosage by gavage in Lonicera japonica treatment groups with Lonicera japonica extract every day. Control group and aging group treated with the same amount of normal saline. 6 weeks later, col ected mice serum samples and various tissue samples for biochemical indicators of SOD, MDA, GSH-Px detection. [Results]After 42days, it appeared different degrees of moving slowly and fur tarnish in aging mice. Aging mice group weight was less than the normal group. Serum, brain, liver and kidney tissue SOD activity, GSH-Px levels were lower than the normal group; serum, liver and kidney tissue MDA levels were higher than the normal group( P <0.05 or P<0.01);These data il ustrated the aging model was general y successful. Compared with the aging mice group, the indicators of Lonicera japonica treatment groups had been improved significantly. Lonicera japonica treatment groups weight were higher than the aging group( P<0.01);serum, liver, kidney SOD activities and GSH-Px levels were higher than the aging group(P<0.05 or P<0.01);MDA levels were lower than the aging group(P<0.05 or P<0.01).[Conclusion] Lonicera japonica extract could resist the oxidation process in D-galactose induced aging model mice, and the middle dose group(5g·kg-1) effect was remarkable.