浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
3期
316-320,326
,共6页
葛姗姗%朱英%郭鑫艳%施思
葛姍姍%硃英%郭鑫豔%施思
갈산산%주영%곽흠염%시사
红腺忍冬叶%抗氧化有效部位%总多酚%含量测定%Folin-Ciocaileu法
紅腺忍鼕葉%抗氧化有效部位%總多酚%含量測定%Folin-Ciocaileu法
홍선인동협%항양화유효부위%총다분%함량측정%Folin-Ciocaileu법
leaves of Lonicera Hypoglauca Miq.%antioxidant effective fractions%total polyphenol%content determination%the method of Folin-Ciocaileu
[目的]建立用Folin-Ciocaileu法测定红腺忍冬叶抗氧化有效部位中总多酚含量的方法,并应用此方法研究不同批次红腺忍冬叶抗氧化有效部位中总多酚含量的变化。[方法]采用Folin-Ciocaileu法,对红腺忍冬叶水提液中总多酚含量测定进行方法学研究,检测12批红腺忍冬叶水提液及其30%乙醇沉淀物和滤液中总多酚的含量。[结果]测得总多酚含量在6μg·mL-1~16μg·mL-1范围内具有良好的线性关系(r=0.9996),重复性RSD为0.31%(n=6),平均加样回收率为98.67%,RSD为2.66%(n=9)。不同批次红腺忍冬叶间测得总多酚含量差别较大;同批次红腺忍冬叶中,总多酚含量为水提液>水提液30%乙醇沉淀后滤液>水提液30%乙醇沉淀。[结论]该方法简单、准确、重复性好,可用于测定红腺忍冬叶抗氧化有效部位中总多酚的含量。红腺忍冬叶抗氧化有效部位中总多酚含量为水提液>水提液30%乙醇沉淀后滤液>水提液30%乙醇沉淀。
[目的]建立用Folin-Ciocaileu法測定紅腺忍鼕葉抗氧化有效部位中總多酚含量的方法,併應用此方法研究不同批次紅腺忍鼕葉抗氧化有效部位中總多酚含量的變化。[方法]採用Folin-Ciocaileu法,對紅腺忍鼕葉水提液中總多酚含量測定進行方法學研究,檢測12批紅腺忍鼕葉水提液及其30%乙醇沉澱物和濾液中總多酚的含量。[結果]測得總多酚含量在6μg·mL-1~16μg·mL-1範圍內具有良好的線性關繫(r=0.9996),重複性RSD為0.31%(n=6),平均加樣迴收率為98.67%,RSD為2.66%(n=9)。不同批次紅腺忍鼕葉間測得總多酚含量差彆較大;同批次紅腺忍鼕葉中,總多酚含量為水提液>水提液30%乙醇沉澱後濾液>水提液30%乙醇沉澱。[結論]該方法簡單、準確、重複性好,可用于測定紅腺忍鼕葉抗氧化有效部位中總多酚的含量。紅腺忍鼕葉抗氧化有效部位中總多酚含量為水提液>水提液30%乙醇沉澱後濾液>水提液30%乙醇沉澱。
[목적]건립용Folin-Ciocaileu법측정홍선인동협항양화유효부위중총다분함량적방법,병응용차방법연구불동비차홍선인동협항양화유효부위중총다분함량적변화。[방법]채용Folin-Ciocaileu법,대홍선인동협수제액중총다분함량측정진행방법학연구,검측12비홍선인동협수제액급기30%을순침정물화려액중총다분적함량。[결과]측득총다분함량재6μg·mL-1~16μg·mL-1범위내구유량호적선성관계(r=0.9996),중복성RSD위0.31%(n=6),평균가양회수솔위98.67%,RSD위2.66%(n=9)。불동비차홍선인동협간측득총다분함량차별교대;동비차홍선인동협중,총다분함량위수제액>수제액30%을순침정후려액>수제액30%을순침정。[결론]해방법간단、준학、중복성호,가용우측정홍선인동협항양화유효부위중총다분적함량。홍선인동협항양화유효부위중총다분함량위수제액>수제액30%을순침정후려액>수제액30%을순침정。
[Objective] To establish a Folin-Ciocaileu method for determination of the contents of total hydroxybenzene in antioxidant effective fraction of leaves of Lonicera Hypoglauca Miq. which is used in the exploration of the content changes of total hydroxybenzene in antioxidant effective fraction of leaves of Lonicera Hypoglauca Miq. of different batches. [Methods]The methodology of the method for determination of the contents of total hydroxybenzene in water extraction of leaves of Lonicera Hypoglauca Miq. was investigated by Folin-Ciocaileu method. The contents of total hydroxybenzene in water extractions or the precipitation and filtrate of 30%ethanol precipitating action from 12 batches of leaves of Lonicera Hypoglauca Miq. were determined.[Results] The methodological study showed that the linear range of total hydroxybenzene was 6 μg·mL-1~16 μg·mL-1 (r=0.9996). The repetitiveness RSD of total polyphenol was 0.31%(n=6). In addition, the average recovery of total polyphenol was 98.67%, with RSD 2.66%(n=9). The total hydroxybenzene contents in different batches extraction of leaves of Lonicera Hypoglauca Miq. were quite different. While the contents of total hydroxybenzene in the same leaves of Lonicera Hypoglauca Miq. were: water extraction>the filtrate of 30% ethanol precipitating action>the precipitation of 30% ethanol precipitating action.[Conclusions]The method is simple, accurate, reproducible and suitable for simultaneous determination of the contents of total polyphenol in antioxidant effective fraction of leaves of Lonicera Hypoglauca Miq.. The total hydroxybenzene contents in various extraction of leaves of Lonicera Hypoglauca Miq. were water extraction>the filtrate of 30% ethanol precipitating action>the precipitation of 30% ethanol precipitating action.