CAJ | 학술논문

研究了铜胺氧化酶（CuAO）及其催化产物过氧化氢（H2 O2）在乙烯利诱导蚕豆气孔关闭中的作用。乙烯释放化合物2-氯乙基磷酸（乙烯利）激活了非质体CuAO活性、提高了保卫细胞 H2 O2产生并诱导气孔关闭，乙烯利的上述效应被 CuAO的两种不可逆抑制剂氨基胍（AG）和2-溴乙胺（BEA）抑制。在几种CuAO催化产物中，只有H2 O2能阻止AG或BEA抑制乙烯利诱导气孔关闭。 CuAO的主要底物腐胺（Put）也不抑制乙烯利诱导气孔关闭。研究结果表明，CuAO 催化产生的H2 O2参与了乙烯诱导的蚕豆气孔关闭。
연구료동알양화매（CuAO）급기최화산물과양화경（H2 O2）재을희리유도잠두기공관폐중적작용。을희석방화합물2-록을기린산（을희리）격활료비질체CuAO활성、제고료보위세포 H2 O2산생병유도기공관폐，을희리적상술효응피 CuAO적량충불가역억제제안기고（AG）화2-추을알（BEA）억제。재궤충CuAO최화산물중，지유H2 O2능조지AG혹BEA억제을희리유도기공관폐。 CuAO적주요저물부알（Put）야불억제을희리유도기공관폐。연구결과표명，CuAO 최화산생적H2 O2삼여료을희유도적잠두기공관폐。
The roles of copper amine oxidase (CuAO)and its products hydrogen peroxide (H2O2) during ethylene-induced stomatal closure inVicia faba were investigated.2-chloroethylene phos-phonic acid (ethephon),an ethylene-releasing compound,stimulated apoplast CuAO activity,in-creased H2 O2 production in guard cells and induced stomatal closure.These effects were sup-pressed by aminoguanidine (AG)and 2-bromoethylamine (BEA),two irreversible inhibitors of CuAO.In the metabolized products of CuAO,only H2 O2 could prevent the inhibitory effect of AG or BEA on stomatal closure induced by ethephon.Putrescine (Put),a main substrate of CuAO,did not prevent ethylene-induced stomatal closure.Take together,the present data sug-gest that H2 O2 generated by CuAO involves in ethylene-induced stomatal closure in Vicia faba.