CAJ | 학술논문

为了建立一种直接获得转基因纯系的转化方法，首次开展了农杆菌介导的黄瓜未受精子房培养遗传转化体系研究。试验以除草剂草胺膦为选择标记，胚胎发生阶段，最适除草剂质量浓度为0.5~1.0 mg·L-1，幼苗生根阶段除草剂质量浓度为2.0 mg·L-1；以羧苄青霉素为抑菌剂，最适抑菌质量为450 mg·L-1；最佳浸染方法为OD600=0.3农杆菌工程菌，浸染10 min；共培养时间为3 d；在浸染菌液和共培养基中加入0.01%的silwet-77，有利于获得较多抗性胚胎及抗性植株。该遗传转化体系的建立，为黄瓜转基因方法研究开辟了新途径。
위료건립일충직접획득전기인순계적전화방법，수차개전료농간균개도적황과미수정자방배양유전전화체계연구。시험이제초제초알련위선택표기，배태발생계단，최괄제초제질량농도위0.5~1.0 mg·L-1，유묘생근계단제초제질량농도위2.0 mg·L-1；이최변청매소위억균제，최괄억균질량위450 mg·L-1；최가침염방법위OD600=0.3농간균공정균，침염10 min；공배양시간위3 d；재침염균액화공배양기중가입0.01%적silwet-77，유리우획득교다항성배태급항성식주。해유전전화체계적건립，위황과전기인방법연구개벽료신도경。
A new transformation technology through Agrobacterium tumefaciens mediated cucumber unfertilized ovary cul-ture was studied. Herbicide glufosinate-ammonium was used as the selection marker,the optimum herbicide selection pres-sure during embryogenesis was 0.5-1.0 mg·L-1,and optimum selection pressure during plantlet rooting was 2.0 mg·L-1;the optimum carbenicillin concentration for bacteria control was 450 mg · L-1;the optimum infection method was soaking ovary slices into A. tumefaciens solution with OD600 at 0.3 for 10 min,and then co-cultured for 3 days;0.01%surfactant silwet-77 in bacteria solution and co-culture medium was beneficial for transformation. The establishment of this new technology pro-vided a new way for cucumber transformation research.