CAJ | 학술논문

综述了西瓜转基因常用的方法、目前已获得的转基因西瓜性状以及转基因西瓜安全性等方面的研究进展，并提出了今后进一步的研究方向。西瓜转基因常用方法为叶盘转化法；将外源基因导入到西瓜基因组中常用的方法主要有农杆菌介导法和花粉管介导法；常采用PCR、Southern和Western等方法来检测和鉴定外源基因是否成功整合到西瓜基因组中；目前利用转基因技术改良的西瓜性状主要集中在培育抗病毒、抗枯萎病和耐旱、耐盐碱等方面；对转基因西瓜的安全性研究主要包括生态安全性和食品安全性2个方面。今后应利用转基因技术进一步提高西瓜营养品质、耐贮性和抗冻性等性状，并进一步加强对转基因西瓜安全性的研究。
종술료서과전기인상용적방법、목전이획득적전기인서과성상이급전기인서과안전성등방면적연구진전，병제출료금후진일보적연구방향。서과전기인상용방법위협반전화법；장외원기인도입도서과기인조중상용적방법주요유농간균개도법화화분관개도법；상채용PCR、Southern화Western등방법래검측화감정외원기인시부성공정합도서과기인조중；목전이용전기인기술개량적서과성상주요집중재배육항병독、항고위병화내한、내염감등방면；대전기인서과적안전성연구주요포괄생태안전성화식품안전성2개방면。금후응이용전기인기술진일보제고서과영양품질、내저성화항동성등성상，병진일보가강대전기인서과안전성적연구。
In this paper,the general methods of transgenic watermelon were summarized,the transgenic watermelon suc-cessfully developed currently was introduced,the security concern of transgenic watermelon was reviewed. The further re-search directions of transgenic watermelon were proposed. The general method of transgenic watermelons was leaf disc trans-formation. Agrobacterium-mediated transformation and pollen-tube-mediated transformation were commonly used to intro-duce exogenous gene into the watermelon genome. PCR,Southern and Western methods were used to detect and identify if exogenous genes weresuccessfully integrated into the watermelon genome . Currently the watermelon characters that using transgenic technology to improve mainly included disease resistance,blight resistance,drought resistance and alkali resis-tance,etc. Research on the safety of transgenic watermelons mainly included the ecological safety and food safety. For the transgenic technology of watermelons,it should further be applied to improve the quality of the watermelon nutrition traits, and storage resistance and freeze resistance. And research on the safety of transgenic should be strengthening in the future.