CAJ | 학술논문

采用超声波辅助提取技术，通过单因素试验和L9（34)正交试验，探讨了提取条件对火棘（Pyracantha fortuneana）中多糖类物质提取率的影响，采用苯酚-硫酸法测定火棘中多糖含量，以葡萄糖作为标准对照物，在482 nm处测定吸光度，并计算其含量。结果表明，在火棘多糖超声波最佳提取工艺条件下（提取剂pH为7，30℃下按液料比30：1提取40 min)，火棘果中多糖提取率达2.148%。
채용초성파보조제취기술，통과단인소시험화L9（34)정교시험，탐토료제취조건대화극（Pyracantha fortuneana）중다당류물질제취솔적영향，채용분분-류산법측정화극중다당함량，이포도당작위표준대조물，재482 nm처측정흡광도，병계산기함량。결과표명，재화극다당초성파최가제취공예조건하（제취제pH위7，30℃하안액료비30：1제취40 min)，화극과중다당제취솔체2.148%。
The conditions of ultrasound-assisted extracting polysaccharides from Pyracantha fortuneana were studied with single factor analysis and L9 (34) orthogonal experiment. The content of polysaccharides in Pyracantha fortuneana was determined by phenol-H2SO4 colorimetry at 482 nm with glucose as the standard. The results showed that the optimal conditions of ultrasonic extracting polysaccharides from Pyracantha fortuneana were 30℃ for 40 min with pH 7 and the best ratio of liquid (mL) to solid(g) was 30:1.Under the optimal conditions,the yield of polysaccharides from Pyracantha fortuneana was 2.148%.