中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2014年
2期
145-149
,共5页
张宝燕%袁静%李向红%吕有勇
張寶燕%袁靜%李嚮紅%呂有勇
장보연%원정%리향홍%려유용
胃肿瘤%淋巴管癌栓%免疫组织化学%D2-40%CKpan%淋巴结转移%预后
胃腫瘤%淋巴管癌栓%免疫組織化學%D2-40%CKpan%淋巴結轉移%預後
위종류%림파관암전%면역조직화학%D2-40%CKpan%림파결전이%예후
Stomach neoplasms%Lymphatic emboli%Immunohistochemistry%D2-40%CKpan%Lymph node metastasis%Prognosis
目的 对比应用D2-40/CKpan免疫组织化学双标记染色(双标记)和苏木精-伊红染色检测胃癌淋巴管癌栓及其预后的临床意义.方法 收集2001年1-12月间于解放军总医院病理科行胃癌根治术的108例进展期胃癌的组织蜡块,以苏木精-伊红染色为对照,评估D2-40/CKpan双标记对淋巴管癌栓的检出情况,并分析其与淋巴结转移及患者总体生存的关系.结果 苏木精-伊红染色检出淋巴管癌栓57例(52.8％),D2-40/CKpan双标记检出淋巴管癌栓73例(67.6％),后者检出率明显高于前者(P=0.007)；苏木精-伊红染色中8例为假阳性,24例为假阴性.经苏木精-伊红染色评估的淋巴管癌栓不仅与淋巴结转移有关(P=0.024),还与患者的总体生存有关(P=0.043)；但经D2-40/CKpan双标记评估的淋巴管癌栓则与淋巴结转移及预后均无关(P=-0.422和P=0.402).结论 D2-40/CKpan双标记提高了淋巴管癌栓的检出率,但基于其评估的淋巴管癌栓与患者淋巴结转移及预后均无关.
目的 對比應用D2-40/CKpan免疫組織化學雙標記染色(雙標記)和囌木精-伊紅染色檢測胃癌淋巴管癌栓及其預後的臨床意義.方法 收集2001年1-12月間于解放軍總醫院病理科行胃癌根治術的108例進展期胃癌的組織蠟塊,以囌木精-伊紅染色為對照,評估D2-40/CKpan雙標記對淋巴管癌栓的檢齣情況,併分析其與淋巴結轉移及患者總體生存的關繫.結果 囌木精-伊紅染色檢齣淋巴管癌栓57例(52.8％),D2-40/CKpan雙標記檢齣淋巴管癌栓73例(67.6％),後者檢齣率明顯高于前者(P=0.007)；囌木精-伊紅染色中8例為假暘性,24例為假陰性.經囌木精-伊紅染色評估的淋巴管癌栓不僅與淋巴結轉移有關(P=0.024),還與患者的總體生存有關(P=0.043)；但經D2-40/CKpan雙標記評估的淋巴管癌栓則與淋巴結轉移及預後均無關(P=-0.422和P=0.402).結論 D2-40/CKpan雙標記提高瞭淋巴管癌栓的檢齣率,但基于其評估的淋巴管癌栓與患者淋巴結轉移及預後均無關.
목적 대비응용D2-40/CKpan면역조직화학쌍표기염색(쌍표기)화소목정-이홍염색검측위암림파관암전급기예후적림상의의.방법 수집2001년1-12월간우해방군총의원병이과행위암근치술적108례진전기위암적조직사괴,이소목정-이홍염색위대조,평고D2-40/CKpan쌍표기대림파관암전적검출정황,병분석기여림파결전이급환자총체생존적관계.결과 소목정-이홍염색검출림파관암전57례(52.8％),D2-40/CKpan쌍표기검출림파관암전73례(67.6％),후자검출솔명현고우전자(P=0.007)；소목정-이홍염색중8례위가양성,24례위가음성.경소목정-이홍염색평고적림파관암전불부여림파결전이유관(P=0.024),환여환자적총체생존유관(P=0.043)；단경D2-40/CKpan쌍표기평고적림파관암전칙여림파결전이급예후균무관(P=-0.422화P=0.402).결론 D2-40/CKpan쌍표기제고료림파관암전적검출솔,단기우기평고적림파관암전여환자림파결전이급예후균무관.
Objective To investigate the evaluation of lymphatic emboli by D2-40/CKpan dual immunostain and its prognostic significance in advanced primary gastric adenocarcinoma.Methods Tissue samples of 108 cases of advanced gastric adenocarcinoma with radical gastrectomy were collected from Chinese PLA General Hospital in 2001.Lymphatic emboli were evaluated by D2-40/CKpan dual immunostain on consecutive sections,and compared with routine HE staining.The correlation of lymphatic emboli with lymph node metastasis and overall survival was analyzed by Pearson Chi-squared test and univariate survival analysis,respectively.Results Lymphatic emboli were detected in 73/108(67.6％) cases by D2-40/CKpan staining,compared to 57/108 (52.8％) by HE staining.There was significant difference (P=0.007).Lymphatic emboli on HE staining revealed false negative in 24/108 cases and false positive in 8/108 cases.A significant correlation was found between lymphatic emboli and lymph node metastasis on HE staining(P=0.024),while it was not found on D2-40/CKpan staining(P=0.422).The overall survival rate was significantly different between lymphatic emboli positive and negative cases on HE staining (P=0.043).The overall survival rate was lower in lymphatic emboli positive cases on HE staining.Lymphatic emboli evaluated on D2-40/CKpan staining had no prognostic value (P=0.402).Conclusion D2-40/CKpan dual immunostaining is more sensitive for lymphatic emboli in gastric adenocarcinoma but may not predict lymph node metastasis and survival,while HE staining may.
