CAJ | 학술논문

通过在pH 3．0的高氯酸溶液中Fenton体系直接氧化罗丹明B产生化学发光，探讨了影响化学发光反应的因素，建立了微量Fe（II）离子的化学发光分析方法．结果表明：该法测定Fe（Ⅱ）离子的浓度线性范围为3．0×10-7～1．2×10-3 mol· L-1，检出限为1．7×10-7 mol· L-1．9次平行测定含Fe（II）离子0．35μg· mL-1的标准溶液的相对标准偏差为4．7％．根据其化学发光光谱、紫外-可见吸收光谱和荧光光谱，推测其发光机理为Fenton体系中产生的羟自由基进攻罗丹明B的1位C原子形成的活性中间体退激发光．
통과재pH 3．0적고록산용액중Fenton체계직접양화라단명B산생화학발광，탐토료영향화학발광반응적인소，건립료미량Fe（II）리자적화학발광분석방법．결과표명：해법측정Fe（Ⅱ）리자적농도선성범위위3．0×10-7～1．2×10-3 mol· L-1，검출한위1．7×10-7 mol· L-1．9차평행측정함Fe（II）리자0．35μg· mL-1적표준용액적상대표준편차위4．7％．근거기화학발광광보、자외-가견흡수광보화형광광보，추측기발광궤리위Fenton체계중산생적간자유기진공라단명B적1위C원자형성적활성중간체퇴격발광．
Rhodamine B was oxidized in Fenton system in HClO 4 solution under the pH of 3.0 accompanied with chemiluminescence .The influencing factors of chemiluminescence of rhodamine B were discussed and a chemiluminescent method for the determination of trace Fe ( II) was developed .The chemiluminescence intensity is linear with the Fe ( II) concentrations in the range of 3.0 ×10 -7 to 1.2 ×10 -3 mol· L-1 , with a detection limit of 1.7 ×10 -7 mol· L-1 .The relative standard deviation (RSD) for the 9 independent detections of 0.35 μg· mL-1 Fe(II) is 4.7%.Based on the chemiluminescence spectra , UV-Vis spectra and fluorescence spectra , it was supposed that hydroxyl radicals produced in Fenton system can attack the carbon atom at the 1st position in the rhodamine B molecule to form an active intermediate , which stimulates luminescence back .