CAJ | 학술논문

本文以苹果腐烂病菌为指示菌，采用菌丝生长速率方法测定补骨脂的抑菌活性，其中提取物采用浸渍法提取，通过反复柱层析、薄层层析等分离纯化方法，对补骨脂粗提物的活性成分进行系统的分离，最终得到5种活性物质，经波谱分析法鉴定5种化合物分别为补骨脂酚（ bakuchiol ）、呋喃香豆素精（bakuchicin）、补骨脂查尔酮（bavachalcone）、补骨脂定（psoralidin）和补骨脂二氢黄酮（bavachin）。经过室内毒力试验，5种化合物的EC50分别为6.117、60.441、3.420、36.815和6.144 mg·L?1，EC90分别为204.480、97.795、15.334、221.860和89.631 mg·L?1，表明5种化合物对苹果腐烂病菌均具有很高的抑制活性。
본문이평과부란병균위지시균，채용균사생장속솔방법측정보골지적억균활성，기중제취물채용침지법제취，통과반복주층석、박층층석등분리순화방법，대보골지조제물적활성성분진행계통적분리，최종득도5충활성물질，경파보분석법감정5충화합물분별위보골지분（ bakuchiol ）、부남향두소정（bakuchicin）、보골지사이동（bavachalcone）、보골지정（psoralidin）화보골지이경황동（bavachin）。경과실내독력시험，5충화합물적EC50분별위6.117、60.441、3.420、36.815화6.144 mg·L?1，EC90분별위204.480、97.795、15.334、221.860화89.631 mg·L?1，표명5충화합물대평과부란병균균구유흔고적억제활성。
The inhibitory activity of Psoralea corylifolia L. extracted by immersion method against Valsa mali was tested. The extracts of P. corylifolia were repeatedly separated and purified by column chromatography, thin layer chromatography and so on, and five compounds were identified as bakuchiol, bavachalcone, bavachin, bakuchicin and psoralidin by spectrum analysis. The antifungal activity of five compounds were tested in vitro and they showed high inhibiting activity to the growth of the hyphae, with the EC50 values were 6.117, 60.441, 3.420, 36.815, 6.144 mg·L?1 and the EC90 values were 204.480, 97.795, 15.334, 221.860, 89.631 mg·L?1 to V. mali, respectively.