CAJ | 학술논문

本研究采用同源重组的方法对产酶溶杆菌Lysobacter enzymogenes OH11中双精氨酸转运系统关键基因tatC进行缺失突变，通过对突变体表型及表型相关基因表达分析来研究该基因在菌株OH11中的功能，重点分析该基因与产酶溶杆菌中热稳定抗真菌因子HSAF生物合成的关系。研究结果表明，基因tatC的突变显著提高了 HSAF 生物合成的产量，以及 HSAF 生物合成的关键基因 pks/nrps 转录水平；改变了菌株OH11的细胞形态，使细胞从棍棒状变为椭圆状；并降低了菌株 OH11生物膜的形成和滑动能力以及在营养缺陷型（10%TSB）培养基中的生长速率，但不改变其在营养丰富培养基（100%TSB）中的生长速率。此外，与野生型OH11相比，基因tatC突变株不改变其蛋白酶、纤维素酶、β?1,3?葡聚糖酶和几丁质酶的产生水平以及其对瓜果腐霉Pythium apanidermatum、立枯丝核菌Rhizoctonia solani和禾谷镰刀菌Fusarium graminearum的拮抗能力。
본연구채용동원중조적방법대산매용간균Lysobacter enzymogenes OH11중쌍정안산전운계통관건기인tatC진행결실돌변，통과대돌변체표형급표형상관기인표체분석래연구해기인재균주OH11중적공능，중점분석해기인여산매용간균중열은정항진균인자HSAF생물합성적관계。연구결과표명，기인tatC적돌변현저제고료 HSAF 생물합성적산량，이급 HSAF 생물합성적관건기인 pks/nrps 전록수평；개변료균주OH11적세포형태，사세포종곤봉상변위타원상；병강저료균주 OH11생물막적형성화활동능력이급재영양결함형（10%TSB）배양기중적생장속솔，단불개변기재영양봉부배양기（100%TSB）중적생장속솔。차외，여야생형OH11상비，기인tatC돌변주불개변기단백매、섬유소매、β?1,3?포취당매화궤정질매적산생수평이급기대과과부매Pythium apanidermatum、립고사핵균Rhizoctonia solani화화곡렴도균Fusarium graminearum적길항능력。
Twin-arginine translocation gene (tatC) of Lysobacter enzymogenes strain OH11 was knocked out by double-cross homologue recombination. Functions of tatC were investigated by comparisons of phenotypes and phenotype-associated gene expressions between wild type and tatC mutant, more attentions were paid on the relationship between tatC and HSAF, an antifungal secondary metabolite produced by L. enzymogenes. Compared with wild-type OH11, mutation of tatC significantly increased the production of HSAF and the transcriptional expression level of pks/nrps, which was a key gene responsible for HSAF biosynthesis. It was observed that the normal clavated cell morphology was changed into ellipticity when disruption of tatC in L. enzymogenes. Furthermore, mutation of tatC remarkably impacted the growth rate in nutrient-limiting broth, biofilm formation and gliding motility. However,MtatC mutation of tatC did not impact the growth rate in nutrient?rich broth, four?type lytic enzyme production, and inhibitory activities against Pythium apanidermatum, Rhizoctonia solani and Fusarium graminearum.