CAJ | 학술논문

为了研究绿僵菌毒力相关的功能基因，采用农杆菌介导的转化方法将携带苯菌灵抗性标记基因的T-DNA导入绿僵菌IMI330189中，获得了绿僵菌突变体库，采用人工饲料诱饵法测定了突变株对3龄东亚飞蝗Locusta migratoria的毒力。研究发现，突变株对东亚飞蝗的毒力都有不同程度的改变，在诱饵剂浓度为108孢子?g?1下，野生型菌株IMI330189对东亚飞蝗的致死中时（LT50）为4.3 d；突变株189-1的毒力略有提高，其LT50值为3.5 d；其余突变株的毒力均降低，且突变株189-4、189-5、189-6、189-10、189-11、189-13和189-15几乎丧失了对东亚飞蝗的毒性，校正死亡率小于50%。本研究为进一步分离毒力相关基因、探索绿僵菌的致病机制奠定了基础。
위료연구록강균독력상관적공능기인，채용농간균개도적전화방법장휴대분균령항성표기기인적T-DNA도입록강균IMI330189중，획득료록강균돌변체고，채용인공사료유이법측정료돌변주대3령동아비황Locusta migratoria적독력。연구발현，돌변주대동아비황적독력도유불동정도적개변，재유이제농도위108포자?g?1하，야생형균주IMI330189대동아비황적치사중시（LT50）위4.3 d；돌변주189-1적독력략유제고，기LT50치위3.5 d；기여돌변주적독력균강저，차돌변주189-4、189-5、189-6、189-10、189-11、189-13화189-15궤호상실료대동아비황적독성，교정사망솔소우50%。본연구위진일보분리독력상관기인、탐색록강균적치병궤제전정료기출。
In order to explore the functional genes of Metarhizium anisopliae related to virulence, a T-DNA containing benomyl resistance gene benA3 was transferred into M. anisopliae strain IMI330189 to construct mutant library by using Agrobacterium-mediated transformation method. The toxicity of some mutants to the third instar larvae of Locusta migratoria was tested at a concentration of 108 spores·g?1 with artificial bait method. The toxicity of mutant 189-1 was slightly shorter with the LT50 3.5 d compared to the wild strain IMI330189 with LT50 4.3 d. The mutants 189-4, 189-5, 189-6, 189-10, 189-11, 189-13 and 189-15 almost lost their toxicity to locust, the corrected mortality rates were less than 50%. By constructing mutant library of M. anisopliae strains IMI330189, we obtained some mutants with virulence lack. The study provided a tool to isolate virulent genes and explore the pathogenic mechanism of M. anisopliae.