CAJ | 학술논문

丽蝇蛹集金小蜂是一类重要的天敌昆虫，大量地应用于生物防治中。虽然寄生蜂不是化学农药的直接作用靶标，但其在自然环境中可能接触到化学农药。P450基因家族是昆虫产生抗药性的重要原因之一，同时也参与了昆虫激素的合成与降解。因此，研究 P450基因家族对充分利用丽蝇蛹集金小蜂，以及保护其免受农药毒杀具有重要的意义。本文利用生物学信息方法从丽蝇蛹集金小蜂基因组筛选到91个P450基因。家族分析表明，丽蝇蛹集金小蜂具有15个P450基因超家族、27个亚家族。对P450基因的正选择和基因转换开展了进一步分析，研究发现5个基因族存在正选择位点，2个基因族存在基因转换，正选择和基因转换之间存在明显的关联性。各个亚家族的选择压力分析表明，CYP9P 亚家族受到选择压力最大（ω=118.9355）。通过MEME程序在丽蝇蛹集金小蜂P450基因上识别到5个频率为90%以上的motif，其中“LAE[NH]P”为新发现的motif。
려승용집금소봉시일류중요적천활곤충，대량지응용우생물방치중。수연기생봉불시화학농약적직접작용파표，단기재자연배경중가능접촉도화학농약。P450기인가족시곤충산생항약성적중요원인지일，동시야삼여료곤충격소적합성여강해。인차，연구 P450기인가족대충분이용려승용집금소봉，이급보호기면수농약독살구유중요적의의。본문이용생물학신식방법종려승용집금소봉기인조사선도91개P450기인。가족분석표명，려승용집금소봉구유15개P450기인초가족、27개아가족。대P450기인적정선택화기인전환개전료진일보분석，연구발현5개기인족존재정선택위점，2개기인족존재기인전환，정선택화기인전환지간존재명현적관련성。각개아가족적선택압력분석표명，CYP9P 아가족수도선택압력최대（ω=118.9355）。통과MEME정서재려승용집금소봉P450기인상식별도5개빈솔위90%이상적motif，기중“LAE[NH]P”위신발현적motif。
Nasonia vitripennisis is an important class of natural enemies of pests, which has been widely used in biological control. Though N. vitripennisis is not the direct target of insecticides, it is still likely to get in touch with insecticides in wide environment. P450 gene family is not only an important factor conferring insecticide resistance but also participates in synthesis and degradation of hormones. Therefore, investigation of P450 genes in N. vitripennisis is great helpful for fully using in biological control and protects it from insecticides. We identified 91 P450 genes from the genome of N. vitripennis. Gene family analysis demonstrated that there were 15 P450 super gene families and 27 subfamilies in N. vitripennis. We also found that five P450 gene groups meet positive selection and two gene groups have gene conversion. There is a positive relation between selection and gene conversion. The results indicated that CYP9P had the highest selection pressure. We also identified five motifs in N. vitripennis P450 gene family using MEME program. The occurrence frequencies of these five motifs were>90%. The“LAE[NH]P”was a new motif identified in N. vitripennis P450 gene family.