F0 F1-ATPase分子马达技术对阪崎肠杆菌检测的研究
F0 F1-ATPase분자마체기술대판기장간균검측적연구
A Rapid Detecting Method for Enterobacter sakazakii based on F0 F1-ATPase Molecular Motor Biosensor
  • 万方数据
    生物技术进展 생물기술진전

    2013年 4期 297-300 ,共4页

    张捷%王煜%陆琳%刘艳华%周琦%孙梓晏%顾德周%尚世进%张惠媛%王佩荣%陈广全%乐加昌

    장첩%왕욱%륙림%류염화%주기%손재안%고덕주%상세진%장혜원%왕패영%진엄전%악가창

    F0 F1-ATPase分子马达%阪崎肠杆菌%ITS探针%快速检测 F0 F1-ATPase分子馬達%阪崎腸桿菌%ITS探針%快速檢測
    F0 F1-ATPase분자마체%판기장간균%ITS탐침%쾌속검측
    F0 F1-ATPase molecular motor%Enterobacter sakazakii%ITS probe%rapid detection
    本研究建立了应用F0 F1-ATPase分子马达生物传感器快速检测阪崎肠杆菌的方法。自嗜热菌中提取载色体后,合成针对阪崎肠杆菌的生物素化ITS探针,在载色体ATP合酶的ε亚基上连接ε亚基抗体-生物素-链霉亲和素-生物素-ITS探针,将待测阪崎肠杆菌标准菌株和阴性对照分别与此生物传感器结合,比较其催化ATP产生量,进而对阪崎肠杆菌DNA进行检测。结果表明,chro ITS探针浓度0.019 mg/mL,阪崎肠杆菌DNA浓度40 ng/mL为最适检测条件。通过与传统检测方法及PCR检测方法对照,本方法具有良好的检测符合性。
    본연구건립료응용F0 F1-ATPase분자마체생물전감기쾌속검측판기장간균적방법。자기열균중제취재색체후,합성침대판기장간균적생물소화ITS탐침,재재색체ATP합매적ε아기상련접ε아기항체-생물소-련매친화소-생물소-ITS탐침,장대측판기장간균표준균주화음성대조분별여차생물전감기결합,비교기최화ATP산생량,진이대판기장간균DNA진행검측。결과표명,chro ITS탐침농도0.019 mg/mL,판기장간균DNA농도40 ng/mL위최괄검측조건。통과여전통검측방법급PCR검측방법대조,본방법구유량호적검측부합성。
    A rapid detecting method for Enterobacter sakazakii based on F0 F1-ATPase molecular motor biosensor was cons tructed. Specific ITS probe were connected with F0 F1-ATPase’s ε subunit by using avidin-biotin system, and then biosensors were combined with the test samples and negative sample, respectively. To compare the catalytic ATP synthesis, Enterobacter sakazakii DNA in the samples were tested. The result showed that the optimum conditions for detection were the concentration of chro ITS of 0. 019 mg/mL and Enterobacter sakazakii DNA concentration of 40 ng/mL. The result was consistent to that of the traditional detection methods and PCR detection method, showing this is in good agreement.
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