CAJ | 학술논문

aiiA基因指导表达的AiiA蛋白是一类能水解细菌群体感应信号分子内酯键的内酯酶。本研究使用CodonW、CUSP和CHIPS等相关程序对苏云金芽胞杆菌中aiiA基因的密码子使用情况进行分析,比较了aiiA基因与大肠杆菌、苏云金芽胞杆菌、枯草芽胞杆菌以及毕赤酵母的密码子偏好性。结果表明,aiiA基因偏好使用以A、T结尾的密码子；aiiA基因的密码子用法与大肠杆菌、苏云金芽胞杆菌、枯草芽胞杆菌和毕赤酵母的基因组密码子用法都有不同程度的偏好差异,其中与毕赤酵母的密码子用法差异最大；比较了aiiA基因分别以苏云金芽胞杆菌、大肠杆菌、枯草芽胞杆菌及毕赤酵母为宿主时密码子使用频率,发现都含有不同程度的低频密码子聚集现象,如果要在上述几种表达系统中实现aiiA基因的高效表达则需对aiiA基因的部分密码子进行优化改造。
aiiA기인지도표체적AiiA단백시일류능수해세균군체감응신호분자내지건적내지매。본연구사용CodonW、CUSP화CHIPS등상관정서대소운금아포간균중aiiA기인적밀마자사용정황진행분석,비교료aiiA기인여대장간균、소운금아포간균、고초아포간균이급필적효모적밀마자편호성。결과표명,aiiA기인편호사용이A、T결미적밀마자；aiiA기인적밀마자용법여대장간균、소운금아포간균、고초아포간균화필적효모적기인조밀마자용법도유불동정도적편호차이,기중여필적효모적밀마자용법차이최대；비교료aiiA기인분별이소운금아포간균、대장간균、고초아포간균급필적효모위숙주시밀마자사용빈솔,발현도함유불동정도적저빈밀마자취집현상,여과요재상술궤충표체계통중실현aiiA기인적고효표체칙수대aiiA기인적부분밀마자진행우화개조。
AiiA, encoded by aiiA gene is one of the homoserine lactone enzyme that can hydrolyze the AHL on the lactone ring of Gram-negative bacteria. Coding sequence of aiiA gene from Bacillus thuringiensis was analyzed by CodonW, CUSP and CHIPS programs for identifying codon bias. Comparing the codon usage of aiiA gene with genomic codon usage of Escherichia coli, Bacillus thuringiensis, Bacillus subtilis and Pichia pastoris, it was indicated that aiiA gene prefers to use the codons ended with A or T;codon bias of aiiA gene from Bacillus thuringiensis was significantly different from those of E. coli, Bacillus subtilis and Pichia pastoris. The result of comparing the usage frequency of aiiA gene in four different hosts showed that there were different degrees of low frenquency regions. The analytic result was very important guideline for designing the aiiA gene to improve the expression of AiiA in those four express systems.