CAJ | 학술논문

本实验利用有机溶剂从葡萄籽中提取原花青素，通过正交实验确定葡萄籽原花青素提取条件为：提取液有机酸调节pH值为3，料液比1∶12，乙醇浓度70%，提取温度70℃，提取时间为2 h，提取3次。提取液通过静态试验从8种大孔吸附树脂中筛选出HPD100型树脂，实验证明该型号树脂对葡萄籽原花青素吸附量大、解吸率高、解吸液纯度高，利于葡萄籽原花青素的分离与纯化。动态实验确定最佳吸附上样浓度为8.0 mg/mL，上样pH值为3，上样流度为3 BV/h。解吸时用60%乙醇2 BV/h流速进行洗脱，通过该工艺方法获得的原花青素纯度达到60%以上。
본실험이용유궤용제종포도자중제취원화청소，통과정교실험학정포도자원화청소제취조건위：제취액유궤산조절pH치위3，료액비1∶12，을순농도70%，제취온도70℃，제취시간위2 h，제취3차。제취액통과정태시험종8충대공흡부수지중사선출HPD100형수지，실험증명해형호수지대포도자원화청소흡부량대、해흡솔고、해흡액순도고，리우포도자원화청소적분리여순화。동태실험학정최가흡부상양농도위8.0 mg/mL，상양pH치위3，상양류도위3 BV/h。해흡시용60%을순2 BV/h류속진행세탈，통과해공예방법획득적원화청소순도체도60%이상。
Organic solvents were used to extract proanthocyanidins from grape seed in this experiment and the extraction conditions were determined by orthogonal experiments as follows:the extraction solution pH value adjusted to 3, material liquid 1:12, 70% ethanol concentration, extraction temperature 70 ℃, extraction time 2 h, extracting 3 times. HPD100 resin was selected from eight kinds of macroporous adsorption resins by static experiment. The experiment results showed that the adsorption quantity and desorption rate of proanthocyanidins and the desorption liquid purity were high with HPD100 resin, good for the separation and purification of grape seed procyanidins. Dynamic experiment determined that the optimal concentration of sample loading was 8.0 mg/mL, pH value of 3, velocity of 3 BV/h. The desorption rate was the highest by 60%ethanol solution with the velocity of 2 BV/h. The purity of proanthocyanidins extracted by this technology was above 60%.