CAJ | 학술논문

目的：改进容量超负荷大鼠模型制备的方法并探讨超声心动图观察大鼠容量超负荷心力衰竭模型心功能状况的准确性与可靠性。方法采用大鼠腹主动脉下腔静脉造瘘方法建立容量超负荷心力衰竭模型，同时设置对照组，造瘘组40只，对照组20只，应用心脏彩色超声诊断仪动态检测大鼠左室舒张末内径（LVEDD）、左室收缩末内径（LVESD）、左房内径（LAD）及左室射血分数（LVEF）等指标，颈动脉插管测定两组大鼠颈动脉血压（CABP）、左心室收缩末期压力（LVSP）和舒张末期压力（LVDP），以及反映左心室收缩功能指标的±dp/dtmax值，以评价两组间血流动力学改变；并于术后12周比较两组大鼠心脏质量及心脏重体重比（心体比）。结果造模术后8周，造瘘组大鼠超声心动图表现为左室心腔扩大呈球形，与假手术组比较，射血分数和短轴缩短率明显降低[LVEF：（61.31±3.07）%vs.（77.20±2.41）%；短轴缩短率：（30.37±2.39）%vs.（41.08±2.13）%]，左室重量指数增加[（2.85±0.41） vs.（1.59±0.15）]，心体比升高[（5.89±0.56） vs.（2.99±0.21）]，差异均有统计学意义（P均＜0.01）；造模术后12周，造瘘组大鼠LVDP[（12.53±22.63）mmHg vs.（2.27±1.77）mmHg]和-dp/dtmax[（-3.78±2.14） vs.（-5.80±2.26）]较假手术组升高，LVSP[（108.88±34.39）mmHg vs.（126.48±19.44）mmHg]和+dp/dtmax[（4.89±2.93） vs.（6.92±1.65）]较假手术组降低（P＜0.05）。结论大鼠超声心动图可较好地动态评价腹主动脉下腔静脉瘘方法建立的容量超负荷心力衰竭模型的心脏形态和心功能状态。
목적：개진용량초부하대서모형제비적방법병탐토초성심동도관찰대서용량초부하심력쇠갈모형심공능상황적준학성여가고성。방법채용대서복주동맥하강정맥조루방법건립용량초부하심력쇠갈모형，동시설치대조조，조루조40지，대조조20지，응용심장채색초성진단의동태검측대서좌실서장말내경（LVEDD）、좌실수축말내경（LVESD）、좌방내경（LAD）급좌실사혈분수（LVEF）등지표，경동맥삽관측정량조대서경동맥혈압（CABP）、좌심실수축말기압력（LVSP）화서장말기압력（LVDP），이급반영좌심실수축공능지표적±dp/dtmax치，이평개량조간혈류동역학개변；병우술후12주비교량조대서심장질량급심장중체중비（심체비）。결과조모술후8주，조루조대서초성심동도표현위좌실심강확대정구형，여가수술조비교，사혈분수화단축축단솔명현강저[LVEF：（61.31±3.07）%vs.（77.20±2.41）%；단축축단솔：（30.37±2.39）%vs.（41.08±2.13）%]，좌실중량지수증가[（2.85±0.41） vs.（1.59±0.15）]，심체비승고[（5.89±0.56） vs.（2.99±0.21）]，차이균유통계학의의（P균＜0.01）；조모술후12주，조루조대서LVDP[（12.53±22.63）mmHg vs.（2.27±1.77）mmHg]화-dp/dtmax[（-3.78±2.14） vs.（-5.80±2.26）]교가수술조승고，LVSP[（108.88±34.39）mmHg vs.（126.48±19.44）mmHg]화+dp/dtmax[（4.89±2.93） vs.（6.92±1.65）]교가수술조강저（P＜0.05）。결론대서초성심동도가교호지동태평개복주동맥하강정맥루방법건립적용량초부하심력쇠갈모형적심장형태화심공능상태。
Objective To improve the method of establishing the rat model of volume-overloaded heart failure, and discuss the accuracy and reliability of echocardiography in observing heart function in rats with volume-overloaded heart failure. Methods The rat model of volume-overloaded heart failure was established by using abdominal aortocaval shunts (model group, n=40), and a sham-operation group was set (n=20). The changes of LVEDD, LVESD, LAD and LVEF were detected by using color echocardiography. The changes of CABP, LVSP, LVDP and ±dp/dtmax were detected by using carotid artery intubation for reviewing hemodynamic changes in two groups. The heart mass indexes and ratio of heart weight to body weight were compared between two groups after the operation for 12 weeks. Results After modeling for 8 weeks, the outcomes of echocardiography showed left ventricular globular dilation in model group. Compared with sham-operation group, LVEF and LVFS decreased significantly [LVEF: (61.31±3.07)% vs. (77.20±2.41)%;LVFS: (30.37±2.39)% vs. (41.08±2.13)%], LVMI increased [(2.85±0.41) vs. (1.59±0.15)],ratio of heart weight to body weight increased [(5.89±0.56) vs. (2.99± 0.21)] in model group (all P<0.01). After modeling for 12 weeks, LVDP [(12.53±22.63) mmHg vs. (2.27±1.77) mmHg] and-dp/dtmax [(-3.78±2.14) vs. (-5.80±2.26)] increased, and LVSP [(108.88±34.39) mmHg vs. (126.48 ±19.44) mmHg] and+dp/dtmax [(4.89±2.93) vs. (6.92±1.65)] decreased in model group compared with sham-operation group (P<0.05). Conclusion Echocardiography can review dynamically the cardiac morphology and heart function of rat model of volume-overloading heart failure established by using abdominal aortocaval shunts.