CAJ | 학술논문

目的：观察吗啡戒断大鼠中脑腹侧背盖区生长相关蛋白-43（growth associated protein-43，GAP-43）在不同时程中的表达，探讨GAP-43在吗啡戒断记忆中的作用。方法采用腹腔递增注射盐酸吗啡并自然戒断的方法建立吗啡依赖1周、2周和4周戒断模型，运用免疫组织化学染色观察中脑腹侧背盖区GAP-43的蛋白表达，并用Image-Pro Plus 5.1图像分析软件对结果进行分析。结果中脑腹侧背盖区GAP-43的蛋白表达随依赖时间的延长表现为先降低再升高（P<0.01）。结论 GAP-43可能在中脑腹侧背盖区参与了吗啡戒断记忆。
목적：관찰마배계단대서중뇌복측배개구생장상관단백-43（growth associated protein-43，GAP-43）재불동시정중적표체，탐토GAP-43재마배계단기억중적작용。방법채용복강체증주사염산마배병자연계단적방법건립마배의뢰1주、2주화4주계단모형，운용면역조직화학염색관찰중뇌복측배개구GAP-43적단백표체，병용Image-Pro Plus 5.1도상분석연건대결과진행분석。결과중뇌복측배개구GAP-43적단백표체수의뢰시간적연장표현위선강저재승고（P<0.01）。결론 GAP-43가능재중뇌복측배개구삼여료마배계단기억。
Objective To observe the protein expression of grow thassociated protein-43 (GAP-43) in mid-brain ventral tegmental area in morphine withdrawal rats at different time, and to evaluate the effect of GAP-43 on morphine withdrawal memory. Methods Rat models of morphine dependent 1 week, 2 weeks and 4 weeks were established by morphine hydrochloride intraperitoneal injection with increasing doses to establish natural withdrawal. The protein expression of GAP-43 in midbrain ventral tegmental area was observed by im munohistochemical staining and the results were analyzed by Im age-Pro Plus 5.1 im-age analysis system . Results With prolongation of dependent time, the expression of GAP-43 was de-creased then increased in midbrain ventral tegmental area . Conclusion GAP-43 could play arole in morphine withdrawal memory in midbrain ventral tegmental area.