CAJ | 학술논문

淡绿叶突变体HM14是通过EMS诱变籼稻IR64获得的，整个生育期叶色均保持淡绿。与野生型相比，突变体除了株高显著降低外，其他农艺性状未发生显著改变。光合色素含量和光合指数测定结果表明，突变体的叶绿素 a、b 含量较野生型 IR64明显下降，突变体的净光合速率显著降低，但蒸腾速率明显上升，光合作用受到显著影响。遗传分析结果表明，突变体淡绿叶性状受一对隐性核基因控制。构建突变体 HM14与Moroberekan的F2分离群体作为基因定位群体，将该淡绿叶基因pglHM14定位在第11染色体短臂 RM1812和 RM26092两个标记之间大约299 kb区间内。
담록협돌변체HM14시통과EMS유변선도IR64획득적，정개생육기협색균보지담록。여야생형상비，돌변체제료주고현저강저외，기타농예성상미발생현저개변。광합색소함량화광합지수측정결과표명，돌변체적협록소 a、b 함량교야생형 IR64명현하강，돌변체적정광합속솔현저강저，단증등속솔명현상승，광합작용수도현저영향。유전분석결과표명，돌변체담록협성상수일대은성핵기인공제。구건돌변체 HM14여Moroberekan적F2분리군체작위기인정위군체，장해담록협기인pglHM14정위재제11염색체단비 RM1812화 RM26092량개표기지간대약299 kb구간내。
The pale-green leaf mutant HM14 was isolated from an EMS-induced IR64 mutant bank.The mutant showed a stable pale-green leaf phenotype throughout the whole life cycle.The plant height of HM14 decreased significantly while other agronomic traits were largely similar to the wild-type IR64.The chlorophyll content of HM14 reduced notably at tillering stage when compared with IR64.The net photosynthetic rate of HM14 was significantly lower than the wild-type IR64 at filling stage while the transpiration rate was raised significantly.Genetic analysis showed that the pale green leaf phenotype was controlled by a single recessive gene.To determine the location of the gene,bulked segregant analysis was carried out using both the mutant and wild-type DNA pools.The gene pglHM14 was finally delimited to a region of 299 kb between SSR markers RM1812 and RM26092 on the short arm of chromosome 11.