CAJ | 학술논문

杂交稻的大青棵现象给我国水稻生产带来了巨大的经济损失,但大青棵产生的遗传基础相关研究尚未见报道。利用蜀恢527/辐恢838群体中的38个导入系为父本,以四个核心不育系II-32A、协青早A、冈46A和金23A为母本,构建4个测交群体。在合肥、杭州和广州3个晚稻环境下,对其进行抽穗期和产量相关性状表型评价,结合基因型分析,利用单向(one-way ANOVA)和双向方差分析(two-way ANOVA)检测与感光性相关的QTL及QTL间互作。表型分析表明,55个组合在合肥表现为大青棵,相比之下,这些组合在杭州和广州表现为正常抽穗,但抽穗期延迟。利用单向方差分析,在多个环境或者群体中检测到12个标记与感光性 QTL紧密连锁。通过双向方差分析,共检测到31对感光性上位性 QTL,其中4对在不同群体和环境下稳定表达。结合基因型分析和上位性分析,发现位点 RM331-RM2、RM331-RM5346、RM3395-RM16和RM3325-RM53间互作是导致导入系 WD57、WD71、WD77、WD78、WD80、WD85和 WD88与4个不育系测交组合产生大青棵的主要遗传基础。
잡교도적대청과현상급아국수도생산대래료거대적경제손실,단대청과산생적유전기출상관연구상미견보도。이용촉회527/복회838군체중적38개도입계위부본,이사개핵심불육계II-32A、협청조A、강46A화금23A위모본,구건4개측교군체。재합비、항주화엄주3개만도배경하,대기진행추수기화산량상관성상표형평개,결합기인형분석,이용단향(one-way ANOVA)화쌍향방차분석(two-way ANOVA)검측여감광성상관적QTL급QTL간호작。표형분석표명,55개조합재합비표현위대청과,상비지하,저사조합재항주화엄주표현위정상추수,단추수기연지。이용단향방차분석,재다개배경혹자군체중검측도12개표기여감광성 QTL긴밀련쇄。통과쌍향방차분석,공검측도31대감광성상위성 QTL,기중4대재불동군체화배경하은정표체。결합기인형분석화상위성분석,발현위점 RM331-RM2、RM331-RM5346、RM3395-RM16화RM3325-RM53간호작시도치도입계 WD57、WD71、WD77、WD78、WD80、WD85화 WD88여4개불육계측교조합산생대청과적주요유전기출。
The problem of abnormal heading in hybrid rice caused great economic losses in recent years.The genetic basis of this phenomenon is still ambiguous and not documented up to now.Four test-crossing populations were constructed using 38 introgression lines (ILs)from Shuhui 527/Fuhui 838 population as male parents and four male sterile lines (II-32A,XieqingzaoA,Gang 46A and Jin 23A)as female parents.The phenotypic evaluation was conducted in Hefei,Hangzhou and Guangzhou in late season.QTL associated with photosensitivity (PS)was identified using one-way analysis of variance (ANOVA)and two-way ANOVA methods with the genotypic and phenotypic data. Progeny testing showed that 55 combinations did not head in Hefei,but normally headed with delayed heading date in Hangzhou and Guangzhou.A total of 12 QTL for PS was found in more than two environments or populations.Using two-way ANOVA,31 epistatic QTLs affecting PS were detected in Hangzhou and Guangzhou,five of which were stably expressed in different populations and environments.The loci interaction between RM331-RM2,RM331-RM5346,RM3395-RM16 and RM3325-RM53 played an important role in controlling the formation of Daqingke for combinations from test-crossing WD57,WD71,WD77,WD78,WD80,WD85 and WD88 with four-types of male sterile lines.