CAJ | 학술논문

Dorsalin-1是骨形态发生蛋白(bone morphogenetic proteins, BMPs)家族成员,在调控脊椎动物神经系统的发育中具有重要作用。目前,国内外有关脊椎动物 Dorsalin-1的研究报道十分有限。本研究克隆了半滑舌鳎(Cynoglossus semilaevis) Dorsalin-1-like基因的cDNA序列,并分析了Dorsalin-1-like mRNA在半滑舌鳎成鱼的组织表达差异及其在早期发育阶段的表达水平变化。研究结果表明,半滑舌鳎Dorsalin-1-like基因cDNA全长为1961 bp,包括了153 bp的5′非翻译区(5′UTR)、编码419个氨基酸的1260 bp的开放读码框(ORF),以及548 bp 的3′非翻译区(3′UTR)。同源性分析和分子进化聚类分析结果显示,半滑舌鳎Dorsalin-1-like 鲀与红鳍东方(Taleifugu rubripes)、尼罗罗非鱼(Oreochromis niloticus)等的同源性最高,并与之共同聚为鱼类 Dorsalin-1分支。此外, Dorsalin-1-like mRNA在半滑舌鳎成鱼的13个组织中具有广泛的分布,并在心脏组织中表达量最高,在肝、脊髓等组织中次之。在半滑舌鳎早期发育阶段(卵期、仔鱼、稚鱼、幼鱼)中, Dorsalin-1-like在卵期中的胚胎期表达量最高,其次为前期仔鱼期。上述实验结果表明, Dorsalin-1在调控卵期和仔鱼期的早期发育中具有重要作用,并可能通过调节神经系统的发育进而参与心脏、骨骼等组织的发育调控。本研究旨在揭示 Dorsalin-1-like 基因在调控半滑舌鳎早期神经系统发育的重要作用,并为筛选半滑舌鳎骨骼发育的调控因子奠定理论基础。
Dorsalin-1시골형태발생단백(bone morphogenetic proteins, BMPs)가족성원,재조공척추동물신경계통적발육중구유중요작용。목전,국내외유관척추동물 Dorsalin-1적연구보도십분유한。본연구극륭료반활설탑(Cynoglossus semilaevis) Dorsalin-1-like기인적cDNA서렬,병분석료Dorsalin-1-like mRNA재반활설탑성어적조직표체차이급기재조기발육계단적표체수평변화。연구결과표명,반활설탑Dorsalin-1-like기인cDNA전장위1961 bp,포괄료153 bp적5′비번역구(5′UTR)、편마419개안기산적1260 bp적개방독마광(ORF),이급548 bp 적3′비번역구(3′UTR)。동원성분석화분자진화취류분석결과현시,반활설탑Dorsalin-1-like 돈여홍기동방(Taleifugu rubripes)、니라라비어(Oreochromis niloticus)등적동원성최고,병여지공동취위어류 Dorsalin-1분지。차외, Dorsalin-1-like mRNA재반활설탑성어적13개조직중구유엄범적분포,병재심장조직중표체량최고,재간、척수등조직중차지。재반활설탑조기발육계단(란기、자어、치어、유어)중, Dorsalin-1-like재란기중적배태기표체량최고,기차위전기자어기。상술실험결과표명, Dorsalin-1재조공란기화자어기적조기발육중구유중요작용,병가능통과조절신경계통적발육진이삼여심장、골격등조직적발육조공。본연구지재게시 Dorsalin-1-like 기인재조공반활설탑조기신경계통발육적중요작용,병위사선반활설탑골격발육적조공인자전정이론기출。
As a member of the bone morphogenetic protein (BMP) family, Dorsalin-1 plays an important role in regulating the development of the nervous system. To date, studies involving the vetebrate Dorsalin-1 has thus far only rarely been reported. In this study, molecular cloning and expression analysis of Dorsalin-1 gene were performed in the tongue sole (Cynoglossus semilaevis). As a result, the Dorsalin-1-like cDNA sequence is 1 961 bp, which contains a 5’-untranslated region (UTR) of 153 bp, an open reading frame (ORF) of 1 260 bp encoding a protein of 419 amino acids, and a 3’-untranslated region (UTR) of 548 bp. The homology and phylogenetic analysis revealed that the deduced amino acid sequence of C. semilaevis Dorsalin-1-like gene exhibited the highest identity with those of Takifugu rubripes and Oreochromis niloticus, and the fish Dorsalin-1 formed a single lineage from the other vertebrates. The tissue expression distribution pattern of Dorsalin-1-like gene was examined in the 13 tissues of female and male adults. The results showed that the Dorsalin-1-like mRNA was predominantly expressed in heart, and less expressed in the liver and spinal cord. Finally, the Dorsalin-1-like mRNA levels at early developmental stages were also detected at egg, larva, juvenile and fingerling stages. The highest level was observed in the embryonic stage (egg stage) and early-stage larvae (larva stage). These findings suggest that Dorsalin-1 may play an important role in regulating early development of the egg and larva stages, and maybe involved in the heart and skeletal development through the regulation of the nervous system.