CAJ | 학술논문

目的：建立快速、灵敏的同时测定人血浆中异烟肼、吡嗪酰胺和利福平的质量浓度的高效液相色谱法。方法血浆样品采用甲醇沉淀蛋白法萃取，采用C18色谱柱（150mm×4．6mm，5．0μm），用甲醇、乙腈、0.05mol·L-1KH2PO3水溶液（三乙胺调pH6.0）进行梯度洗脱，柱温40℃，进样量20μL，采用PDA紫外检测器。结果以峰面积外标法定量，异烟肼在0．20～25．6μg·mL-1范围内，与峰面积线性关系良好（r＝0．9998），吡嗪酰胺在0．20～25．6μg·mL-1范围内，与峰面积线性关系良好（r＝0．9999），利福平胶囊在0．40～25．6μg·mL-1范围内，与峰面积线性关系良好（r＝0．9996），回收率为85％～115％，日内、日间精密度RSD均＜9．4％（n＝5）。结论该方法专属性强、灵敏、准确，适用于同时测定人血浆样品中异烟肼、吡嗪酰胺和利福平的质量浓度。
목적：건립쾌속、령민적동시측정인혈장중이연정、필진선알화리복평적질량농도적고효액상색보법。방법혈장양품채용갑순침정단백법췌취，채용C18색보주（150mm×4．6mm，5．0μm），용갑순、을정、0.05mol·L-1KH2PO3수용액（삼을알조pH6.0）진행제도세탈，주온40℃，진양량20μL，채용PDA자외검측기。결과이봉면적외표법정량，이연정재0．20～25．6μg·mL-1범위내，여봉면적선성관계량호（r＝0．9998），필진선알재0．20～25．6μg·mL-1범위내，여봉면적선성관계량호（r＝0．9999），리복평효낭재0．40～25．6μg·mL-1범위내，여봉면적선성관계량호（r＝0．9996），회수솔위85％～115％，일내、일간정밀도RSD균＜9．4％（n＝5）。결론해방법전속성강、령민、준학，괄용우동시측정인혈장양품중이연정、필진선알화리복평적질량농도。
Objective To develop an HPLC method for the simultaneous determination of isoniazid ,rifampicin and pyrazinamide in human plasma .Methods The compounds and internal standard were extracted from the plasma by a protein precipitation procedure with methanol .The separation of the analytes was performed on a C18 column (150 mm × 4 .6 mm ,5 .0μm) at 40 ℃ ,using a gra-dient elution program with solvents consisting of methanol ,acetonitrile and 0.05 mol · L -1 KH2 PO3-H2O .The injection volume was 20 μL .The compounds were detected by PDA .Results Calibration curves in spiked plasma were linear at 0 .20-25 .6 ,0.20-25 .6 and 0 .40-25 .6 μg · mL - 1 for isoniazid ,rifampicin and pyrazinamide ,respectively .The recoveries were 85% ~115% ,and the intra-day and inter-day RSDs were <9 .4% .Conclusion The method is specific ,sensitive and accurate ,and it can be used for the simultaneous determination of isoniazid ,rifampicin and pyrazinamide in human plasma .