CAJ | 학술논문

目的：观察中药复方胶囊（YSYN）对大鼠多发性脑梗死痴呆（MID）的疗效及作用机制。方法于左侧颈外动脉注入复合血栓诱导剂，复制多发性脑梗死痴呆模型。采用Morris水迷宫法进行学习记忆测试；采用酶联免疫法测定海马组织去甲肾上腺素（NE）水平；采用比色法测定海马组织乙酰胆碱酯酶（AchE）、乙酰胆碱转移酶（CHAT）和谷胱甘肽过氧化物酶（GSH-Px）水平。结果 YSYN能够显著提高多发性脑梗死痴呆大鼠学习记忆能力（P＜0．05）；显著降低多发性脑梗死痴呆大鼠海马组织AchE含量，提高多发性脑梗死痴呆大鼠海马组织CHAT、NE及GSH-Px含量（P＜0．05～0．01）。结论 YSYN能够显著改善多发性脑梗死痴呆大鼠的学习记忆功能，对MID具有较好的保护作用，其作用机制可能是通过调节海马组织内NE、Ach（乙酰胆碱）及GS H （谷胱甘肽）的活性来实现的。
목적：관찰중약복방효낭（YSYN）대대서다발성뇌경사치태（MID）적료효급작용궤제。방법우좌측경외동맥주입복합혈전유도제，복제다발성뇌경사치태모형。채용Morris수미궁법진행학습기억측시；채용매련면역법측정해마조직거갑신상선소（NE）수평；채용비색법측정해마조직을선담감지매（AchE）、을선담감전이매（CHAT）화곡광감태과양화물매（GSH-Px）수평。결과 YSYN능구현저제고다발성뇌경사치태대서학습기억능력（P＜0．05）；현저강저다발성뇌경사치태대서해마조직AchE함량，제고다발성뇌경사치태대서해마조직CHAT、NE급GSH-Px함량（P＜0．05～0．01）。결론 YSYN능구현저개선다발성뇌경사치태대서적학습기억공능，대MID구유교호적보호작용，기작용궤제가능시통과조절해마조직내NE、Ach（을선담감）급GS H （곡광감태）적활성래실현적。
Objective To investigate the effect of Chinese herbal compound caspsules (YSYN ) on the model rats of multi-infarct dementia(MID) and its mechanism .Methods The multi-infarct dementia model was induced by injecting compound thrombosis in-ducer into the external carotid artery .Morris water maze test was used to evaluate the abilities of learning-memory ;the level of norepinephrine(NE) in hippocampal tissue were assayed by using enzyme-linked immunsorbent ;the levels of acetylcholinesterase (AchE) ,cholinacetyltrans-ferase(CHAT) and glutathione peroxidase(GSH-Px) in the hippocampal tissue were assayed by using colorimetry .Results The YSYN could significantly improved the abilities of learning-memory in MID rats(P<0 .05);descreased the level of AchE in hippocampal tissue ,increased the level of CHAT ,NE and GSH-Px in hippocampal tissue(P<0 .05-0 .01) . Conclusion The YSYN could significantly improved the abilities of learning-memory in MID rats ,has a good protective effect on MID rats ;its mechanism may be related to adjusting the level of NE ,AchE ,CHAT and GSH-Px in hippocampal tissue .