CAJ | 학술논문

目的：建立一种同时测定复方炔雌醇甲羟孕酮胶囊中维生素A、维生素D2和维生素E含量的高效液相色谱方法。方法色谱柱为Kromasil C18柱，流动相为甲醇-乙腈（90∶10），检测波长为265 nm。结果维生素A、维生素D2和维生素E检测质量浓度的线性范围分别为0．1～1．0mg·mL -1（r＝0．9997）、0．2～5．0μg·mL -1（r＝0．9997）和0．5～16mg·mL -1（r＝0.9999），平均回收率分别为99．6％，100．0％和100．9％，RSD分别为1．0％，1．1％和0．9％。结论该方法快速、准确、重复性好，可用于复方炔雌醇甲羟孕酮胶囊中3种维生素的含量测定。
목적：건립일충동시측정복방결자순갑간잉동효낭중유생소A、유생소D2화유생소E함량적고효액상색보방법。방법색보주위Kromasil C18주，류동상위갑순-을정（90∶10），검측파장위265 nm。결과유생소A、유생소D2화유생소E검측질량농도적선성범위분별위0．1～1．0mg·mL -1（r＝0．9997）、0．2～5．0μg·mL -1（r＝0．9997）화0．5～16mg·mL -1（r＝0.9999），평균회수솔분별위99．6％，100．0％화100．9％，RSD분별위1．0％，1．1％화0．9％。결론해방법쾌속、준학、중복성호，가용우복방결자순갑간잉동효낭중3충유생소적함량측정。
Objective To develop an HPLC method for the simultaneous determination of vitamin A ,vitamin D2 and vitamin E in Compound Ethinyloestradiol and Medroxyprogesterone Capsules .Methods The determination was performed on a Kromasil C18 column ,with a mobile phase consisted of methanol-acetonitrile (90∶10) ,and the detection wavelength was set at 265 nm . Re-sults The linear range of vitamin A ,vitamin D2 and vitamin E were 0 .1~1 .0 mg · mL -1 (r=0 .999 7) ,0 .2~5 .0μg · mL -1 (r=0 .999 7) and 0 .5~16 mg · mL -1 (r=0 .999 9) ,and the average recovery rate were 99 .6% ,100 .0% and 100 .9% ,with RSD of 1 .0% ,1 .1% and 0 .9% ,respectively .Conclusion The established method is easy to operate and shows good precision and repro-ducibility ,and could be used for the determination of 3 kinds of vitamins in Ethinyloestradiol and Medroxyprogesterone Capsules .