CAJ | 학술논문

目的：建立高效液相色谱-蒸发光检测法测定白头翁皂苷D含量的方法。方法 Hyperisil ODS2 C18柱（250 mm ×4．6 mm ，5μm），柱温30℃；流动相：甲醇-水-甲酸（72∶28∶0．1）；流速1 mL · min-1；Altech ELSD 3300检测器，雾化气流速1．3 L · min-1，漂移管温度为55℃。结果该方法的线性范围为0．1046～1．0460 mg · mL -1（r＝0．9992），平均回收率（n＝6）为101．0％（RSD＝1．6％），不同产地药材白头翁皂苷D差异性较大。结论该方法专属性强、准确、灵敏度高、重复性好，可用于白头翁中白头翁皂苷D的测定，对白头翁规范性种植具有指导意义。
목적：건립고효액상색보-증발광검측법측정백두옹조감D함량적방법。방법 Hyperisil ODS2 C18주（250 mm ×4．6 mm ，5μm），주온30℃；류동상：갑순-수-갑산（72∶28∶0．1）；류속1 mL · min-1；Altech ELSD 3300검측기，무화기류속1．3 L · min-1，표이관온도위55℃。결과해방법적선성범위위0．1046～1．0460 mg · mL -1（r＝0．9992），평균회수솔（n＝6）위101．0％（RSD＝1．6％），불동산지약재백두옹조감D차이성교대。결론해방법전속성강、준학、령민도고、중복성호，가용우백두옹중백두옹조감D적측정，대백두옹규범성충식구유지도의의。
Objective To establish a determination method of pulsatilla saponin D in Pulsatilla chinensis by HPLC-ELSD method . Methods Samples were analyzed on a Hyperisil ODS2 C18 column(250 mm × 4 .6 mm ,5μm) and methanol-water-formic acid(72∶28∶0 .1) was used as the mobile phase .The flow rate was 1 mL · min-1 and the temperature was 30 ℃ .The Altech ELSD 3300 detector was uesd as a detector .The flow rate of atomizer was 1 .3 L · min-1 and the temperature of drift tube was 55 ℃ .Result The method had a linear relationship in the range of 0 .104 6-1 .046 0 mg · mL -1 (r=0 .999 2) and the average recovery rate was 101.0% (n=6 ,RSD 1 .6% ) .The contents of pulsatilla saponin D were much different in different areas .Conclusion The method has advantages of high specific ,accurate ,sensitive and reproducible ,and can be used for the determination of pulsatilla saponin D in Pulsatilla chinensis ,which has a pioneer significance for Pulsatilla chinensis standard planting .