CAJ | 학술논문

也页目的：探讨藕节对糖尿病肾病大鼠肾组织p-JAK2、p-STAT3及凋亡因子Bcl-2、Bax表达的影响。方法：健康雄性SD大鼠60只,随机选取10只为正常组( N组),其余采用单次腹腔注射链尿佐菌素( STZ,45 mg/kg)制作DN模型,造模成功后随机分为糖尿病肾病组(DN)、藕节小剂量组(RL,1.5 g·kg-1·d-1)、中剂量组(RM,3.0 g·kg-1·d-1)、大剂量组(RH,6.0 g·kg-1·d-1)及氯沙坦钾组(LP,30 mg·kg-1·d-1),均采用灌胃给药,N组和DN组给予等量蒸馏水。12周后检测大鼠生化指标；HE、Masson染色及电镜观察肾脏病理改变；免疫组化法测定p-JAK2、p-STAT3、Bcl-2及Bax在肾组织表达情况；原位末端标记法( TUNEL)检测肾组织细胞凋亡情况。结果：实验12周末,与N组比较,DN组大鼠肾小球肥大、系膜基质增多、细胞凋亡明显,BUN、Scr、24 h尿蛋白定量明显升高(P<0.05),肾组织Bax、p-JAK2、p-STAT3表达明显上调,Bcl-2表达下调(P<0.05)；与DN组比较,藕节中、高剂量组肾脏病理改变减轻、细胞凋亡减少,24 h尿蛋白定量较DN组明显降低(P<0.05),但降尿蛋白作用弱于氯沙坦钾组,同时肾组织Bax、p-JAK2、p-STAT3表达下调,Bcl-2表达上调(P<0.05)。结论：藕节可能通过上调Bcl-2在肾组织的表达,下调Bax、p-JAK2、p-STAT3的表达,从而减少尿蛋白,延缓DN进展。
야혈목적：탐토우절대당뇨병신병대서신조직p-JAK2、p-STAT3급조망인자Bcl-2、Bax표체적영향。방법：건강웅성SD대서60지,수궤선취10지위정상조( N조),기여채용단차복강주사련뇨좌균소( STZ,45 mg/kg)제작DN모형,조모성공후수궤분위당뇨병신병조(DN)、우절소제량조(RL,1.5 g·kg-1·d-1)、중제량조(RM,3.0 g·kg-1·d-1)、대제량조(RH,6.0 g·kg-1·d-1)급록사탄갑조(LP,30 mg·kg-1·d-1),균채용관위급약,N조화DN조급여등량증류수。12주후검측대서생화지표；HE、Masson염색급전경관찰신장병리개변；면역조화법측정p-JAK2、p-STAT3、Bcl-2급Bax재신조직표체정황；원위말단표기법( TUNEL)검측신조직세포조망정황。결과：실험12주말,여N조비교,DN조대서신소구비대、계막기질증다、세포조망명현,BUN、Scr、24 h뇨단백정량명현승고(P<0.05),신조직Bax、p-JAK2、p-STAT3표체명현상조,Bcl-2표체하조(P<0.05)；여DN조비교,우절중、고제량조신장병리개변감경、세포조망감소,24 h뇨단백정량교DN조명현강저(P<0.05),단강뇨단백작용약우록사탄갑조,동시신조직Bax、p-JAK2、p-STAT3표체하조,Bcl-2표체상조(P<0.05)。결론：우절가능통과상조Bcl-2재신조직적표체,하조Bax、p-JAK2、p-STAT3적표체,종이감소뇨단백,연완DN진전。
Objective:To investigate the effect of Rhizoma Nelumbinis on expression of p-JAK2,p-STAT3,Bcl-2 and Bax in renal tissues of diabetic nephropathy rats. Methods:60 SD male rats were randomly divided into 6 groups:the normal control group(group N),diabetic nephropathic model group(group DN),low-dose Rhizoma Nelumbinis group (RL,1. 5 g·kg-1·d-1), medium-dose Rhizoma Nelumbinis group(RM,3. 0 g·kg-1·d-1),high-dose Rhizoma Nelumbinis group(RH,6. 0 g·kg-1· d-1 ) and losartan potassium group ( LP, 30 mg · kg-1 · d-1 ) . Rats were treated respectively by intragastric administration for 12 weeks. Meanwhile rats of group N and group DN received the same volume distilled water by gavage. Blood serum creatinine,blood urea nitrogen and 24 h urinary protein were measured after the research. The renal pathological changes were observed by histochemis-try staining and electron microscope. The expression of p-JAK2,p-STAT3,Bcl-2 and Bax were detected by immunohistochemis-try. The cell apoptosis was detected by TUNEL. Results:Compared with group N,the glomerular became hypertrophic and the mesang-ial matrix increased in group DN. BUN,Scr,24 h urinary protein also increased significantly (P<0. 05). Meanwhile the expression of Bax,p-JAK2,p-STAT3 in renal tissue increased,the expression of Bcl-2 decreased significantly(P<0. 05). Compared with group DN,group RM and group RH had significantly lower 24 h urinary protein (P<0. 05),the pathology changes were relieved,cell apop-tosis was reduced,and the expression of Bax,p-JAK2,p-STAT3 decreased and the expression of Bcl-2 increased (P<0. 05). In addition,the effect of reducing urinary protein in group RM,RH was less than those of group LP. Conclusion:Rhizoma Nelumbinis may reducing urinary protein and delay the injury in diabetic nephropathy by down-regulating the level of Bax,p-JAK2,p-STAT3, up-regulating the level of Bcl-2.