中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
10期
756-760
,共5页
田新利%王新%胡天驹%白丽莉%张浩%陈宇红%朱萌%赵朕龙%高萌%徐超%胡赞民%彭宜红
田新利%王新%鬍天駒%白麗莉%張浩%陳宇紅%硃萌%趙朕龍%高萌%徐超%鬍讚民%彭宜紅
전신리%왕신%호천구%백려리%장호%진우홍%주맹%조짐룡%고맹%서초%호찬민%팽의홍
NP-1%抗菌机制%MRSA%MDR-PA
NP-1%抗菌機製%MRSA%MDR-PA
NP-1%항균궤제%MRSA%MDR-PA
NP-1%Antibacterial mechanism%MRSA%MDR-PA
目的：初步探讨小球藻表达的兔防御素NP-1的抗菌机制。方法通过液体培养抑制测定法、阳离子中和试验和扫描电镜技术，分别测定小球藻表达的兔防御素NP-1对临床分离的1株耐甲氧西林金黄色葡萄球菌（ MRSA）和1株多重耐药铜绿假单胞菌（ MDR-PA）的抑菌动力学作用、明确正电荷在NP-1抗菌作用中的重要性，以及NP-1对细菌形态结构的影响。结果小球藻表达的兔防御素NP-1对MRSA和MDR-PA的抑菌率在3 h达到80％以上，其中对MRSA的抗菌作用具有浓度依赖性；带有正电荷的精氨酸（0．25％～0．50％）能有效抑制NP-1的抗菌作用；此外，扫描电镜观察到NP-1可分别导致菌体出现孔洞（ MRSA）或变形塌陷（ MDR-PA）。结论小球藻兔防御素NP-1对耐药的革兰阳性菌和革兰阴性菌均有良好的抗菌作用；一定浓度的精氨酸能够竞争性抑制NP-1的抗菌作用；NP-1对MRSA的抗菌机制可能属于“孔洞”学说。
目的：初步探討小毬藻錶達的兔防禦素NP-1的抗菌機製。方法通過液體培養抑製測定法、暘離子中和試驗和掃描電鏡技術，分彆測定小毬藻錶達的兔防禦素NP-1對臨床分離的1株耐甲氧西林金黃色葡萄毬菌（ MRSA）和1株多重耐藥銅綠假單胞菌（ MDR-PA）的抑菌動力學作用、明確正電荷在NP-1抗菌作用中的重要性，以及NP-1對細菌形態結構的影響。結果小毬藻錶達的兔防禦素NP-1對MRSA和MDR-PA的抑菌率在3 h達到80％以上，其中對MRSA的抗菌作用具有濃度依賴性；帶有正電荷的精氨痠（0．25％～0．50％）能有效抑製NP-1的抗菌作用；此外，掃描電鏡觀察到NP-1可分彆導緻菌體齣現孔洞（ MRSA）或變形塌陷（ MDR-PA）。結論小毬藻兔防禦素NP-1對耐藥的革蘭暘性菌和革蘭陰性菌均有良好的抗菌作用；一定濃度的精氨痠能夠競爭性抑製NP-1的抗菌作用；NP-1對MRSA的抗菌機製可能屬于“孔洞”學說。
목적：초보탐토소구조표체적토방어소NP-1적항균궤제。방법통과액체배양억제측정법、양리자중화시험화소묘전경기술，분별측정소구조표체적토방어소NP-1대림상분리적1주내갑양서림금황색포도구균（ MRSA）화1주다중내약동록가단포균（ MDR-PA）적억균동역학작용、명학정전하재NP-1항균작용중적중요성，이급NP-1대세균형태결구적영향。결과소구조표체적토방어소NP-1대MRSA화MDR-PA적억균솔재3 h체도80％이상，기중대MRSA적항균작용구유농도의뢰성；대유정전하적정안산（0．25％～0．50％）능유효억제NP-1적항균작용；차외，소묘전경관찰도NP-1가분별도치균체출현공동（ MRSA）혹변형탑함（ MDR-PA）。결론소구조토방어소NP-1대내약적혁란양성균화혁란음성균균유량호적항균작용；일정농도적정안산능구경쟁성억제NP-1적항균작용；NP-1대MRSA적항균궤제가능속우“공동”학설。
Objective To investigate antibacterial mechanism of rabbit defensin NP-1 expressed by Chlorella.Methods Liquid culture inhibition assay was used for detecting antibacterial effects of rabbit defensin NP-1 on a methicillin-resistant Staphylococcus aureus (MRSA) isolate and one strain of multidrug resistant Pseudomonas aeruginosa ( MDR-PA) .The cation neutralization test was applied to analyze effects of positive charges on the antibacterial activity of NP-1.The scanning electron microscopy was used to observe bacterial morphological structures of MRSA and MDR-PA with NP-1 intervention .Results The antibacteri-al rates of NP-1 against MRSA and MDR-PA reached up to 80%after three hours of intervention .The anti-bacterial activity of NP-1 against MRSA was in a dose-dependent manner .Competitive inhibition assay dem-onstrated that positively charged arginine , with concentrations ranging from 0.25%to 0.50%, could effec-tively inhibit antibacterial activities of NP-1.The scanning electron microscopy showed that NP-1 could in-duce holes on the membrane of MRSA , and deformation or collapse of MDR-PA.Conclusion The rabbit defensin NP-1 expressed by Chlorella has strong antibacterial effects on drug-resistant gram-positive bacteria and gram-negative bacteria , which could be competitively inhibited by a certain concentration of arginine . The antibacterial mechanism of NP-1against MRSA might be related to the pore-forming process.
