CAJ | 학술논문

目的：探讨miR-146a对新生隐球菌诱导 THP-1细胞分泌IL-1β和TNF-α的调控作用。方法采用加热法灭活新生隐球菌，荧光定量 RT-PCR 及 ELISA 检测灭活新生隐球菌经miR-146a促进人THP-1细胞分泌IL-1β和TNF-α的作用，同时了解THP-1细胞Dectin-1和TLR4封闭前后IL-1β和TNF-α水平变化。构建miR-146a mimics和inhibitor转染的THP-1细胞株，观察灭活新生隐球菌诱导不同细胞株分泌IL-1β和TNF-α水平差异。结果灭活新生隐球菌可以上调THP-1细胞miR-146a表达水平，封闭 Dectin -1和 TLR4可使 miR-146a 表达水平下调。 miR-146a mimics 或inhibitor转染后可分别抑制和促进灭活新生隐球菌诱导的THP-1细胞分泌IL-1β和TNF-α能力。结论新生隐球菌可经Dectin-1和TLR4上调THP-1细胞miR-146a表达水平，miR-146a具有负调控新生隐球菌诱导THP-1细胞分泌IL-1β和TNF-α的作用。
목적：탐토miR-146a대신생은구균유도 THP-1세포분비IL-1β화TNF-α적조공작용。방법채용가열법멸활신생은구균，형광정량 RT-PCR 급 ELISA 검측멸활신생은구균경miR-146a촉진인THP-1세포분비IL-1β화TNF-α적작용，동시료해THP-1세포Dectin-1화TLR4봉폐전후IL-1β화TNF-α수평변화。구건miR-146a mimics화inhibitor전염적THP-1세포주，관찰멸활신생은구균유도불동세포주분비IL-1β화TNF-α수평차이。결과멸활신생은구균가이상조THP-1세포miR-146a표체수평，봉폐 Dectin -1화 TLR4가사 miR-146a 표체수평하조。 miR-146a mimics 혹inhibitor전염후가분별억제화촉진멸활신생은구균유도적THP-1세포분비IL-1β화TNF-α능력。결론신생은구균가경Dectin-1화TLR4상조THP-1세포miR-146a표체수평，miR-146a구유부조공신생은구균유도THP-1세포분비IL-1β화TNF-α적작용。
Objective To investigate the regulatory effects of miR-146a on inflammation factors production in C ryptococcus neoformans treated THP-1 cells.Methods Cryptcoo ccus neoformans strains were heat killed .Fluorescence quantitative RTP-CR and ELISA were used to detect the levels of IL -1βand TNF-αin THP-1 cells treated with heat-killed Cryptococcus neoformans.In addition, the production of IL-1βand TNF-αwere analyzed before and after Dectin-1or TLR 4 blocked .THP-1 cell lines that were respectively transfected with miR-146 a mimics and inhibitors were constructed and the production of IL-1βand TNF-αin these cell lines were analyzed after interfered with Cryptococcus neoformans.Results With the interference of heat-killed Cryptococcus neoformans, the expression of miR-146a was up-regulated in THP-1 cells, but was down-regulated after Dectin-1 or TLR4 blocked.The expressions of IL-1βand TNF-αinduced by heat-killed Cryptococcus neoformans were enhanced in miR-146a mimics transfected THP-1 cells, but was inhibi-ted in inhibitors transfected THP-1 cells.Conclusion Heat-killed Cryptococcus neoformans could up-regu-late miR-146a expression in THP-1 cells via Dectin-1 and TLR.miR-146a could negatively regulate the ex-pressions of IL-1βand TNF-αinduced by Cryptococcus neoformans.