CAJ | 학술논문

背景与目的转录因子Sox2维持干细胞的全能性，参与肿瘤干细胞的自我更新，在多种肿瘤的发生发展中发挥重要作用。本研究旨在探讨Sox2及Sox2自身抗体（Sox2-Ab）在非小细胞肺癌（non-small cell lung cancer, NSCLC）患者组织及血清中的表达和意义。方法荧光定量PCR及免疫组化法检测58例NSCLC、16例其他肿瘤和20例正常肺组织标本中Sox2基因及蛋白表达，ELISA法检测30例NSCLC患者和30例健康体检者血清Sox2-Ab水平，结合NSCLC临床病理特点进行数据分析。结果肺癌组织中Sox2 mRNA水平及蛋白阳性表达率均高于其他肿瘤及正常肺组织，差异均有统计学意义（P<0.01），且Sox2 mRNA表达增高同肺癌患者病理类型及肿瘤大小有关，与性别、年龄、肿瘤分化程度和淋巴结转移等无关。血清Sox2-Ab水平在NSCLC患者和正常体检者差异无统计学意义。结论Sox2在NSCLC中有较高的表达，与病理类型、肿瘤大小密切相关，Sox2可能成为肺癌新的标志物及治疗靶点。
배경여목적전록인자Sox2유지간세포적전능성，삼여종류간세포적자아경신，재다충종류적발생발전중발휘중요작용。본연구지재탐토Sox2급Sox2자신항체（Sox2-Ab）재비소세포폐암（non-small cell lung cancer, NSCLC）환자조직급혈청중적표체화의의。방법형광정량PCR급면역조화법검측58례NSCLC、16례기타종류화20례정상폐조직표본중Sox2기인급단백표체，ELISA법검측30례NSCLC환자화30례건강체검자혈청Sox2-Ab수평，결합NSCLC림상병리특점진행수거분석。결과폐암조직중Sox2 mRNA수평급단백양성표체솔균고우기타종류급정상폐조직，차이균유통계학의의（P<0.01），차Sox2 mRNA표체증고동폐암환자병리류형급종류대소유관，여성별、년령、종류분화정도화림파결전이등무관。혈청Sox2-Ab수평재NSCLC환자화정상체검자차이무통계학의의。결론Sox2재NSCLC중유교고적표체，여병리류형、종류대소밀절상관，Sox2가능성위폐암신적표지물급치료파점。
Background and objective Transcription factor Sox2 remains the pluripotency of stem cell, partici-pates in self-renew of cancer stem cell and plays important role during the initiation and development of various cancers. hTis study intends to investigate the expression and signiifcance of Sox2 and Sox2 autoantibodies (Sox2-Ab) in tissue and serum of patients with non-small cell lung cancer (NSCLC). Methods Expression of Sox2 gene and protein was tested in 58 cases of NSCLC, 16 patients with other tumors and 20 cases of normal lung tissue specimens by quantitative PCR and immunohis-tochemical assay, respectively. Serum Sox2-Ab level was detected in 30 cases of NSCLC patients and 30 healthy controls by ELISA method. Clinical and pathological data from patients were collected and analyzed retrospectively. Results Expression levels of Sox2 mRNA and protein were higher in patient with NSCLC than other groups, with statistically signiifcant differenc-es (P<0.01), respectively. Meanwhile, Sox2 mRNA expression increased in NSCLC patients associated with histological type and tumor size. No signiifcant differences in Sox2-Ab serum levels were found between NSCLC patients and normal subjects. Conclusion Sox2 in NSCLC have a higher expression, which is closely related to histological type and tumor size. Sox2 might use as a potential biomarker and therapeutic target for lung cancer.