中国肺癌杂志
中國肺癌雜誌
중국폐암잡지
CHINESE JOURNAL OF LUNG CANCER
2013年
11期
567-571
,共5页
陈献珊%韩坤元%陈锋夏%吴从明%黄伟炜
陳獻珊%韓坤元%陳鋒夏%吳從明%黃偉煒
진헌산%한곤원%진봉하%오종명%황위위
青蒿琥酯%肺肿瘤%侵袭%ICAM-1%MMP-9
青蒿琥酯%肺腫瘤%侵襲%ICAM-1%MMP-9
청호호지%폐종류%침습%ICAM-1%MMP-9
Artesunate%Lung neoplasms%Invasion%ICAM-1%MMP-9
背景与目的近年来研究发现抗疟药青蒿琥酯对肺癌细胞具有抑制效应，但其作用机制尚未完全阐明。本研究旨在探讨青蒿琥酯对肺癌A549细胞侵袭能力的影响。方法 MTT实验观察青蒿琥酯对体外培养的A549细胞增殖抑制作用；Transwell小室侵袭实验检测青蒿琥酯对体外A549细胞侵袭转移能力影响；构建A549细胞裸鼠皮下移植瘤模型，观察青蒿琥酯对A549细胞移植瘤的抑制作用，并采用Western blot检测A549细胞移植瘤ICAM-1和MMP-9蛋白表达的变化。结果 MTT实验表明1.25μg/L-5μg/L的低浓度青蒿琥酯对A549细胞增殖无明显抑制作用。Transwell小室体外侵袭实验表明，与对照组（96.33±6.41）比较，1.25μg/L青蒿琥酯即可抑制A549细胞侵袭转移（75.43±4.37, P<0.05）。移植瘤实验表明10 mg/kg青蒿琥酯不能抑制裸鼠A549细胞移植瘤的生长（P>0.05），但是可降低ICAM-1和MMP-9蛋白表达量（P<0.05）。结论青蒿琥酯可抑制A549细胞侵袭潜能，其机制可能与降低ICAM-1和MMP-9表达有关。
揹景與目的近年來研究髮現抗瘧藥青蒿琥酯對肺癌細胞具有抑製效應，但其作用機製尚未完全闡明。本研究旨在探討青蒿琥酯對肺癌A549細胞侵襲能力的影響。方法 MTT實驗觀察青蒿琥酯對體外培養的A549細胞增殖抑製作用；Transwell小室侵襲實驗檢測青蒿琥酯對體外A549細胞侵襲轉移能力影響；構建A549細胞裸鼠皮下移植瘤模型，觀察青蒿琥酯對A549細胞移植瘤的抑製作用，併採用Western blot檢測A549細胞移植瘤ICAM-1和MMP-9蛋白錶達的變化。結果 MTT實驗錶明1.25μg/L-5μg/L的低濃度青蒿琥酯對A549細胞增殖無明顯抑製作用。Transwell小室體外侵襲實驗錶明，與對照組（96.33±6.41）比較，1.25μg/L青蒿琥酯即可抑製A549細胞侵襲轉移（75.43±4.37, P<0.05）。移植瘤實驗錶明10 mg/kg青蒿琥酯不能抑製裸鼠A549細胞移植瘤的生長（P>0.05），但是可降低ICAM-1和MMP-9蛋白錶達量（P<0.05）。結論青蒿琥酯可抑製A549細胞侵襲潛能，其機製可能與降低ICAM-1和MMP-9錶達有關。
배경여목적근년래연구발현항학약청호호지대폐암세포구유억제효응，단기작용궤제상미완전천명。본연구지재탐토청호호지대폐암A549세포침습능력적영향。방법 MTT실험관찰청호호지대체외배양적A549세포증식억제작용；Transwell소실침습실험검측청호호지대체외A549세포침습전이능력영향；구건A549세포라서피하이식류모형，관찰청호호지대A549세포이식류적억제작용，병채용Western blot검측A549세포이식류ICAM-1화MMP-9단백표체적변화。결과 MTT실험표명1.25μg/L-5μg/L적저농도청호호지대A549세포증식무명현억제작용。Transwell소실체외침습실험표명，여대조조（96.33±6.41）비교，1.25μg/L청호호지즉가억제A549세포침습전이（75.43±4.37, P<0.05）。이식류실험표명10 mg/kg청호호지불능억제라서A549세포이식류적생장（P>0.05），단시가강저ICAM-1화MMP-9단백표체량（P<0.05）。결론청호호지가억제A549세포침습잠능，기궤제가능여강저ICAM-1화MMP-9표체유관。
Background and objective Artesunate, an anti-malarial drug, elicits an inhibitory effect on pulmonary carcinoma. However, the mechanisms of artesunate activity on pulmonary carcinoma have not been completely elucidated. hTe aim of this study is to investigate the effect of artesunate on the invasion of human lung adenocarcinoma A549 cells. Methods hTe inhibitory effect of artesunate on the proliferation and invasion of A549 cells was determined in vitro by MTT assay and transwell chamber invasion assay, respectively. A nude mouse model of human lung A549 cell xenogratf tumor was established. hTe inhibitory effect of artesunate on the tumor of the mouse model as well as ICAM-1 and MMP-9 protein expressions were determined by Western blot. Results A low dose of artesunate ranging between 1.25μg/L and 5μg/L did not signiifcantly inhibit the proliferation of A549 cells in vitro. By contrast, 1.25μg/L artesunate inhibited the invasion of A549 cells in vitro as determined by transwell chamber invasion assay (96.33±6.41 vs 75.43±4.37, P<0.05). Although 10 mg/kg artesunate did not signiifcantly inhibit A549 xenogratf tumor proliferation (P>0.05), artesunate decreased the ICAM-1 and MMP-9 protein levels in the mouse model (P<0.05). Conclusion Artesunate could inhibit the invasion of human lung adenocarcinoma A549 cells by possibly downregulating ICAM-1 and MMP-9 expressions.
