CAJ | 학술논문

目的：观察足细胞环氧合酶-2（COX-2）基因敲低对足细胞相关蛋白表达的影响。方法：以条件永生性小鼠足细胞株为研究对象，分为足细胞正常对照组、足细胞COX-2基因敲低组和足细胞阴性转染组，用流式细胞仪检测足细胞凋亡水平，用 Western bolt 检测COX-2、Nephrin、Podocin、CD2相关蛋白（CD2AP）的表达水平。结果：与足细胞正常对照组及阴性转染组相比，足细胞COX-2基因敲低组COX-2蛋白表达明显降低、细胞凋亡率明显增高，而 Nephrin、Podocin、CD2AP蛋白表达则显著降低。结论：足细胞 COX-2基因敲低可以导致足细胞凋亡，且足细胞Nephrin、Podocin、CD2AP蛋白的表达下调。
목적：관찰족세포배양합매-2（COX-2）기인고저대족세포상관단백표체적영향。방법：이조건영생성소서족세포주위연구대상，분위족세포정상대조조、족세포COX-2기인고저조화족세포음성전염조，용류식세포의검측족세포조망수평，용 Western bolt 검측COX-2、Nephrin、Podocin、CD2상관단백（CD2AP）적표체수평。결과：여족세포정상대조조급음성전염조상비，족세포COX-2기인고저조COX-2단백표체명현강저、세포조망솔명현증고，이 Nephrin、Podocin、CD2AP단백표체칙현저강저。결론：족세포 COX-2기인고저가이도치족세포조망，차족세포Nephrin、Podocin、CD2AP단백적표체하조。
Objective:To investigate the effect of cyclooxygenase-2 (COX-2) gene knockdown on podocyte associated proteins .Methods:The conditionally immortalized murine podocytes were divided into three groups :nor-mal control group ,podocytes treated with siRNA and podocytes treated with negative siRNA .The level of podocyte apoptosis was measured by flow cytometry .The proteins expression of COX-2、Nephrin、Podocin、CD2AP were detec-ted by western blotting .Results :The expression of COX-2 protein was decreased and the podocytes apoptosis were increased in podocytes treated with siRNA .And the protein expressions of Nephrin、Podocin、CD2AP in siRNA group were significantly lower than those in normal control group and negative siRNA group .Conclusion :The COX-2 gene knockdown induces podocytes apoptosis .The protein expression of Nephrin、Podocin、CD2AP in podocyte treated with siRNA are downregulated .