陕西医学杂志
陝西醫學雜誌
협서의학잡지
SHAANXI MEDICAL JOURNAL
2013年
11期
1443-1445
,共3页
任凯%付慧%陈鹏%成凡%茹晓荣%郭慧芳
任凱%付慧%陳鵬%成凡%茹曉榮%郭慧芳
임개%부혜%진붕%성범%여효영%곽혜방
前列腺肿瘤/生物学%鞘脂激活蛋白/分析%@雄激素受体
前列腺腫瘤/生物學%鞘脂激活蛋白/分析%@雄激素受體
전렬선종류/생물학%초지격활단백/분석%@웅격소수체
Prostatic neoplasms/biology%Sphingolipid activator/analysis%Androgen receptor
目的:探讨Saposin C对LNCaP细胞雄激素受体(AR)蛋白降解的影响。方法:通过转染实验,将Saposin C、TAT-Saposin C真核表达载体转染至LNCaP细胞,MTS检测Saposin C对LNCaP细胞增殖的影响,Western blot检测Saposin C对AR蛋白水平的影响及其在细胞中降解的调节作用。结果:Saposin C抑制LNCaP细胞AR蛋白的降解,上调胞内AR蛋白水平,促进LNCaP细胞增殖。结论:Saposin C增强AR蛋白的稳定性抑制其在细胞中的降解。
目的:探討Saposin C對LNCaP細胞雄激素受體(AR)蛋白降解的影響。方法:通過轉染實驗,將Saposin C、TAT-Saposin C真覈錶達載體轉染至LNCaP細胞,MTS檢測Saposin C對LNCaP細胞增殖的影響,Western blot檢測Saposin C對AR蛋白水平的影響及其在細胞中降解的調節作用。結果:Saposin C抑製LNCaP細胞AR蛋白的降解,上調胞內AR蛋白水平,促進LNCaP細胞增殖。結論:Saposin C增彊AR蛋白的穩定性抑製其在細胞中的降解。
목적:탐토Saposin C대LNCaP세포웅격소수체(AR)단백강해적영향。방법:통과전염실험,장Saposin C、TAT-Saposin C진핵표체재체전염지LNCaP세포,MTS검측Saposin C대LNCaP세포증식적영향,Western blot검측Saposin C대AR단백수평적영향급기재세포중강해적조절작용。결과:Saposin C억제LNCaP세포AR단백적강해,상조포내AR단백수평,촉진LNCaP세포증식。결론:Saposin C증강AR단백적은정성억제기재세포중적강해。
Objective:To investigate the effect of Saposin C on Androgen Receptor protein degradation in LNCaP cells .Methods :After transient transfection of the eukaryotic expression vectors containing Saposin C and TAT-Saposin C into LNCaP cells ,MTS was used to detect effects of Saposin C and TAT-Saposin C on proliferation of LNCaP cells .Western blot assay was used to investigate effects of Saposin C and TAT-Saposin C on AR protein levels .Results :Saposin C and TAT-Saposin C could promote the proliferation of LNCaP cells .Its mechanism was that Saposin C and TAT-Saposin C up-regulated AR protein stability and restrained AR protein degradation .Conclu-sion:Saposin C could enhance AR protein levels and stability .