CAJ | 학술논문

目的：建立气相色谱法测定阿育魏果中麝香草酚的方法。方法采用气相色谱法对样品中麝香草酚进行定量分析，采用Agilent HP-5（30 m ×03．2 mm ，02．5μm）为色谱柱；以50℃作为初始柱温，恒温2 min ，再以10℃/min升温至230℃保持10 min。采用FID检测器，检测器温度为250℃，载气为氮气，柱流速为1 mL/min。结果麝香草酚进样量在浓度为02．0～20．0 mg/mL（r ＝09．999）范围内与其峰面积呈良好线性关系。平均回收率为1004．7％（n ＝6），RSD为16．0％。10批不同产地样品中麝香草酚的含量为18．84％～33．11％，平均含量为24．44％。结论首次用GC色谱法对阿育魏果药材挥发性成分麝香草酚进行测定。GC法简便、精确，为有效制订质量标准提供科学依据。
목적：건립기상색보법측정아육위과중사향초분적방법。방법채용기상색보법대양품중사향초분진행정량분석，채용Agilent HP-5（30 m ×03．2 mm ，02．5μm）위색보주；이50℃작위초시주온，항온2 min ，재이10℃/min승온지230℃보지10 min。채용FID검측기，검측기온도위250℃，재기위담기，주류속위1 mL/min。결과사향초분진양량재농도위02．0～20．0 mg/mL（r ＝09．999）범위내여기봉면적정량호선성관계。평균회수솔위1004．7％（n ＝6），RSD위16．0％。10비불동산지양품중사향초분적함량위18．84％～33．11％，평균함량위24．44％。결론수차용GC색보법대아육위과약재휘발성성분사향초분진행측정。GC법간편、정학，위유효제정질량표준제공과학의거。
Objective To establish a method for determination of the content of thymol in seed of Trachyspermum ammi (L .) Sprague by GC .Methods GC method was used for quantitative analysis of thymol content .It was carried out on Agilent HP-5 column (30 m × 0 3.2 mm ,0 2.5 μm) .The initial tem-perature was set at 50 ℃ ,and held for 2 minutes ,and then programmed to 230 ℃at the rate of 10 ℃/min ,and held for 10 minutes .The detector was FID ,and its temperature was set at 250 ℃ .The carrying gas was nitrogen and its flow rate was 1 0. mL/min .Results There were good linear relationships between the peak area and concentration at the range of 0 2.0-2 0.0 mg/mL for thymol (r =0 9.99 9) .The average recovery rate of thymol was 100 4.7% (n =6) ,with RSD 1 6.0% .The average content of ten different sam-ples was 2 4.4% .Conclusion GC method was used for the first time to determine the volatile components of seed of Trachyspermum ammi (L .) Sprague .The method is simple ,accurate and can provide scientific basis for quality control of seed of Trachyspermum ammi (L .)Sprague .