CAJ | 학술논문

目的：探讨糖原合酶激酶-3β（GSK-3β）、与张力蛋白同源的位于第10号染色体磷酸酶基因（PT EN ）、Polo样激酶1（PLK1）在儿童急性髓系白血病（AML）中的表达及意义。方法 RT-PCR方法检测实验组（33例初诊AML患儿的骨髓）和对照组（10例正常骨髓）骨髓单个核细胞（BMMNC）中GSK-3βmRNA、PTEN mRNA、PLK1 mRNA的表达，ELISA方法检测GSK-3β蛋白及P-GSK-3β的表达。结果实验组中GSK-3β mRNA、GSK-3β蛋白、PLK1 mRNA的表达量高于对照组（P＝0．012；P＝0．014；P＝0．040）；PTEN mRNA、P-GSK-3β的表达量低于对照组（P＝0．012；P＝0．002）。GSK3β蛋白与 PTEN mRNA的表达呈负相关（r＝-0．415，P＝0．016）；GSK-3βmRNA、GSK3β蛋白与 PLK1 mRNA的表达成呈正相关（r＝0．388，P＝0．026；r＝0．427，P＝0．013）。外周血白细胞计数增高者GSK-3β蛋白表达增高；临床危险度高者GSK-3β mRNA、GSK-3β蛋白的表达增高，P-GSK-3β的表达降低。结论在儿童AML中，GSK-3β和PLK1可能起癌基因的作用，PTEN可能起抑癌基因的作用。
목적：탐토당원합매격매-3β（GSK-3β）、여장력단백동원적위우제10호염색체린산매기인（PT EN ）、Polo양격매1（PLK1）재인동급성수계백혈병（AML）중적표체급의의。방법 RT-PCR방법검측실험조（33례초진AML환인적골수）화대조조（10례정상골수）골수단개핵세포（BMMNC）중GSK-3βmRNA、PTEN mRNA、PLK1 mRNA적표체，ELISA방법검측GSK-3β단백급P-GSK-3β적표체。결과실험조중GSK-3β mRNA、GSK-3β단백、PLK1 mRNA적표체량고우대조조（P＝0．012；P＝0．014；P＝0．040）；PTEN mRNA、P-GSK-3β적표체량저우대조조（P＝0．012；P＝0．002）。GSK3β단백여 PTEN mRNA적표체정부상관（r＝-0．415，P＝0．016）；GSK-3βmRNA、GSK3β단백여 PLK1 mRNA적표체성정정상관（r＝0．388，P＝0．026；r＝0．427，P＝0．013）。외주혈백세포계수증고자GSK-3β단백표체증고；림상위험도고자GSK-3β mRNA、GSK-3β단백적표체증고，P-GSK-3β적표체강저。결론재인동AML중，GSK-3β화PLK1가능기암기인적작용，PTEN가능기억암기인적작용。
Objective To explore the expression and clinical significance of GSK-3β,PTEN and PLK1 in pediatric AML . Methods Experiment group was bone marrows of 33 cases incipient children with AML .Control group was 10 cases normal bone marrows .GSK-3β,PTEN and PLK1 gene expressions in BMMNC of the two groups were tested using semi quantitative reverse transcriptase polymerase chain reaction(RT-PCR)technique .GSK-3βprotein and P-GSK-3βexpressions were tested by ELISA .Re-sults The expression of GSK-3βmRNA ,GSK-3βprotein ,PLK1 mRNA in BMMNC of children with AML was higher than that of control group(P=0 .012 ;P= 0 .014 ;P= 0 .040);The expression of PTEN mRNA ,P-GSK-3β was lower than the control group (P=0 .012 ;P=0 .002);GSK-3βprotein had a negative correlation with PTEN mRNA (r= -0 .415 ,P=0 .016);GSK-3βmRNA , GSK-3βprotein had a positive correlation with PLK 1 mRNA(r=0 .388 ,P=0 .026;r=0 .427 ,P=0 .013) .The expression of GSK-3βprotein was high in which had high peripheral white blood cell counts ;both the expressions of GSK-3βmRNA and GSK-3βpro-tein were high in which had high risk ;but the expression of P-GSK-3βwere low .Conclusion In pediatric AML ,GSK-3βand PLK1 may play a role of oncogene and PTEN may play a role of tumor suppressor gene .